Assessment of silver nanoparticle toxicity for common carp
(Cyprinus carpio) fish embryos using a novel method controlling
the agglomeration in the aquatic media

J 2015

Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media

OPRŠAL, Jakub, Luděk BLÁHA, Miloslav POUZAR, Petr KNOTEK, Milan VLČEK et. al.

Basic information

Original name

Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media

Authors

OPRŠAL, Jakub (203 Czech Republic, guarantor), Luděk BLÁHA (203 Czech Republic, belonging to the institution), Miloslav POUZAR (203 Czech Republic), Petr KNOTEK (203 Czech Republic), Milan VLČEK (203 Czech Republic) and Kateřina HRDÁ (203 Czech Republic)

Edition

Environmental Science and Pollution Research, HEIDELBERG (GERMANY), Springer, 2015, 0944-1344

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.760

RIV identification code

RIV/00216224:14310/15:00086677

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s11356-015-5120-4

UT WoS

000365816000077

Keywords in English

Nanosilver; Agglomeration; Fish embryo; Cyprinus carpio; Particle size distribution

Abstract

V originále

Formation of agglomerates and their rapid sedimentation during aquatic ecotoxicity testing of nanoparticles is a major issue with a crucial influence on the risk assessment of nanomaterials. The present work is aimed at developing and testing a new approach based on the periodic replacement of liquid media during an ecotoxicological experiment which enabled the efficient monitoring of exposure conditions. A verified mathematical model predicted the frequencies of media exchanges which checked for formation of agglomerates from silver nanoparticles AgNP with 50 nm average size of the original colloid. In the model experiments, embryos of common carp Cyprinus carpio were exposed repeatedly for 6 h to AgNPs (5-50 mu m Ag L-1) either under semistatic conditions (exchange of media after 6 h) or in variants with frequent media exchanges (varying from 20 to 300 min depending on the AgNP colloid concentration and the desired maximum agglomerate size of 200 or 400 nm). In contrast to other studies, where dissolved free metals are usually responsible for toxic effects, our 144-h experiments demonstrated the importance of AgNP agglomerates in the adverse effects of nanosilver. Direct adsorption of agglomerates on fish embryos locally increased Ag concentrations which resulted in pronounced toxicity particularly in variants with larger 400 nm agglomerates. The present study demonstrates the suitability of the novel methodology in controlling the conditions during aquatic nanomaterial toxicity testing. It further provided insights into the mechanisms underlying the effects of AgNP, which rank on a global scale among the most widely used nanomaterials.

Links

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

OPRŠAL, Jakub, Luděk BLÁHA, Miloslav POUZAR, Petr KNOTEK, Milan VLČEK and Kateřina HRDÁ. Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media. Environmental Science and Pollution Research. HEIDELBERG (GERMANY): Springer, 2015, vol. 22, No 23, p. 19124-19132. ISSN 0944-1344. Available from: https://dx.doi.org/10.1007/s11356-015-5120-4.

@article{1336264,
   author = {Opršal, Jakub and Bláha, Luděk and Pouzar, Miloslav and Knotek, Petr and Vlček, Milan and Hrdá, Kateřina},
   article_location = {HEIDELBERG (GERMANY)},
   article_number = {23},
   doi = {http://dx.doi.org/10.1007/s11356-015-5120-4},
   keywords = {Nanosilver; Agglomeration; Fish embryo; Cyprinus carpio; Particle size distribution},
   language = {eng},
   issn = {0944-1344},
   journal = {Environmental Science and Pollution Research},
   title = {Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media},
   url = {http://link.springer.com/article/10.1007%2Fs11356-015-5120-4},
   volume = {22},
   year = {2015}
}

TY  - JOUR
ID  - 1336264
AU  - Opršal, Jakub - Bláha, Luděk - Pouzar, Miloslav - Knotek, Petr - Vlček, Milan - Hrdá, Kateřina
PY  - 2015
TI  - Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media
JF  - Environmental Science and Pollution Research
VL  - 22
IS  - 23
SP  - 19124-19132
EP  - 19124-19132
PB  - Springer
SN  - 09441344
KW  - Nanosilver
KW  - Agglomeration
KW  - Fish embryo
KW  - Cyprinus carpio
KW  - Particle size distribution
UR  - http://link.springer.com/article/10.1007%2Fs11356-015-5120-4
L2  - http://link.springer.com/article/10.1007%2Fs11356-015-5120-4
N2  - Formation of agglomerates and their rapid sedimentation during aquatic ecotoxicity testing of nanoparticles is a major issue with a crucial influence on the risk assessment of nanomaterials. The present work is aimed at developing and testing a new approach based on the periodic replacement of liquid media during an ecotoxicological experiment which enabled the efficient monitoring of exposure conditions. A verified mathematical model predicted the frequencies of media exchanges which checked for formation of agglomerates from silver nanoparticles AgNP with 50 nm average size of the original colloid. In the model experiments, embryos of common carp Cyprinus carpio were exposed repeatedly for 6 h to AgNPs (5-50 mu m Ag L-1) either under semistatic conditions (exchange of media after 6 h) or in variants with frequent media exchanges (varying from 20 to 300 min depending on the AgNP colloid concentration and the desired maximum agglomerate size of 200 or 400 nm). In contrast to other studies, where dissolved free metals are usually responsible for toxic effects, our 144-h experiments demonstrated the importance of AgNP agglomerates in the adverse effects of nanosilver. Direct adsorption of agglomerates on fish embryos locally increased Ag concentrations which resulted in pronounced toxicity particularly in variants with larger 400 nm agglomerates. The present study demonstrates the suitability of the novel methodology in controlling the conditions during aquatic nanomaterial toxicity testing. It further provided insights into the mechanisms underlying the effects of AgNP, which rank on a global scale among the most widely used nanomaterials.
ER  -

OPRŠAL, Jakub, Luděk BLÁHA, Miloslav POUZAR, Petr KNOTEK, Milan VLČEK and Kateřina HRDÁ. Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media. \textit{Environmental Science and Pollution Research}. HEIDELBERG (GERMANY): Springer, 2015, vol.~22, No~23, p.~19124-19132. ISSN~0944-1344. Available from: https://dx.doi.org/10.1007/s11356-015-5120-4.

Detailed Information on Publication Record