STEUER, A., A. SCHMIDT, Pavel BABICA a J. F. KOLB. Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer. Online. In Jarm, T., Kramar, P. Proceedings of 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies. Springer Singapore, 2016, s. 192-195. ISBN 978-981-287-817-5. Dostupné z: https://dx.doi.org/10.1007/978-981-287-817-5. |
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@inproceedings{1337005, author = {Steuer, A. and Schmidt, A. and Babica, Pavel and Kolb, J. F.}, booktitle = {Proceedings of 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies}, doi = {http://dx.doi.org/10.1007/978-981-287-817-5}, editor = {Jarm, T., Kramar, P.}, keywords = {nanosecond pulsed electric field; gap junctional intercellular communication; connexin 43}, howpublished = {elektronická verze "online"}, isbn = {978-981-287-817-5}, pages = {192-195}, publisher = {Springer Singapore}, title = {Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer}, url = {http://link.springer.com/chapter/10.1007%2F978-981-287-817-5_43}, year = {2016} }
TY - JOUR ID - 1337005 AU - Steuer, A. - Schmidt, A. - Babica, Pavel - Kolb, J. F. PY - 2016 TI - Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer PB - Springer Singapore SN - 9789812878175 KW - nanosecond pulsed electric field KW - gap junctional intercellular communication KW - connexin 43 UR - http://link.springer.com/chapter/10.1007%2F978-981-287-817-5_43 N2 - Nanosecond pulsed electric fields (nsPEFs) can induce different biological effects in cells, depending on pulse length and field strength. Currently, they are investigated for medical applications such as cancer treatment and wound healing. One particular response that has so far not been investigated is the effect on the communication between cells. Gap junctional intercellular communication (GJIC) is important to maintain the homeostasis in tissues and plays a crucial role in many diseases. For the study of the effects of nsPEFs confluent monolayers of rat liver epithelial cells WB-F344 were exposed to 20 pulses of 100 ns and up to 20 kV/cm and GJIC was tested by scrape loading/dye transfer assay. Gap junctions are built from the protein connexin, therefore changes of connexin 43 (Cx43) distribution in the cells were examined by immunofluorescent staining. Cells showed a field strength dependent decreased communication. In addition less Cx43 was detectable in the membrane after exposure. ER -
STEUER, A., A. SCHMIDT, Pavel BABICA a J. F. KOLB. Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer. Online. In Jarm, T., Kramar, P. \textit{Proceedings of 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food \&{} Environmental Technologies}. Springer Singapore, 2016, s.~192-195. ISBN~978-981-287-817-5. Dostupné z: https://dx.doi.org/10.1007/978-981-287-817-5.
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