Effects of nanosecond pulsed electric fields on cell-cell
communication in a monolayer

D 2016

Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer

STEUER, A., A. SCHMIDT, Pavel BABICA a J. F. KOLB

Základní údaje

Originální název

Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer

Autoři

STEUER, A., A. SCHMIDT, Pavel BABICA a J. F. KOLB

Vydání

Proceedings of 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies, od s. 192-195, 2016

Nakladatel

Springer Singapore

Další údaje

Typ výsledku

Stať ve sborníku

Obor

30304 Public and environmental health

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-981-287-817-5

DOI

http://dx.doi.org/10.1007/978-981-287-817-5

Klíčová slova anglicky

nanosecond pulsed electric field; gap junctional intercellular communication; connexin 43

Příznaky

Mezinárodní význam

Změněno: 22. 2. 2016 14:01, Mgr. et Mgr. Pavlína Lolloková

Anotace

V originále

Nanosecond pulsed electric fields (nsPEFs) can induce different biological effects in cells, depending on pulse length and field strength. Currently, they are investigated for medical applications such as cancer treatment and wound healing. One particular response that has so far not been investigated is the effect on the communication between cells. Gap junctional intercellular communication (GJIC) is important to maintain the homeostasis in tissues and plays a crucial role in many diseases. For the study of the effects of nsPEFs confluent monolayers of rat liver epithelial cells WB-F344 were exposed to 20 pulses of 100 ns and up to 20 kV/cm and GJIC was tested by scrape loading/dye transfer assay. Gap junctions are built from the protein connexin, therefore changes of connexin 43 (Cx43) distribution in the cells were examined by immunofluorescent staining. Cells showed a field strength dependent decreased communication. In addition less Cx43 was detectable in the membrane after exposure.

Citovat

STEUER, A., A. SCHMIDT, Pavel BABICA a J. F. KOLB. Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer. Online. In Jarm, T., Kramar, P. Proceedings of 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies. Springer Singapore, 2016, s. 192-195. ISBN 978-981-287-817-5. Dostupné z: https://dx.doi.org/10.1007/978-981-287-817-5.

@inproceedings{1337005,
   author = {Steuer, A. and Schmidt, A. and Babica, Pavel and Kolb, J. F.},
   booktitle = {Proceedings of 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies},
   doi = {http://dx.doi.org/10.1007/978-981-287-817-5},
   editor = {Jarm, T., Kramar, P.},
   keywords = {nanosecond pulsed electric field; gap junctional intercellular communication; connexin 43},
   howpublished = {elektronická verze "online"},
   isbn = {978-981-287-817-5},
   pages = {192-195},
   publisher = {Springer Singapore},
   title = {Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer},
   url = {http://link.springer.com/chapter/10.1007%2F978-981-287-817-5_43},
   year = {2016}
}

TY  - JOUR
ID  - 1337005
AU  - Steuer, A. - Schmidt, A. - Babica, Pavel - Kolb, J. F.
PY  - 2016
TI  - Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer
PB  - Springer Singapore
SN  - 9789812878175
KW  - nanosecond pulsed electric field
KW  - gap junctional intercellular communication
KW  - connexin 43
UR  - http://link.springer.com/chapter/10.1007%2F978-981-287-817-5_43
N2  - Nanosecond pulsed electric fields (nsPEFs) can induce different biological effects in cells, depending on pulse length and field strength. Currently, they are investigated for medical applications such as cancer treatment and wound healing. One particular response that has so far not been investigated is the effect on the communication between cells. Gap junctional intercellular communication (GJIC) is important to maintain the homeostasis in tissues and plays a crucial role in many diseases. For the study of the effects of nsPEFs confluent monolayers of rat liver epithelial cells WB-F344 were exposed to 20 pulses of 100 ns and up to 20 kV/cm and GJIC was tested by scrape loading/dye transfer assay. Gap junctions are built from the protein connexin, therefore changes of connexin 43 (Cx43) distribution in the cells were examined by immunofluorescent staining. Cells showed a field strength dependent decreased communication. In addition less Cx43 was detectable in the membrane after exposure.
ER  -

STEUER, A., A. SCHMIDT, Pavel BABICA a J. F. KOLB. Effects of nanosecond pulsed electric fields on cell-cell communication in a monolayer. Online. In Jarm, T., Kramar, P. \textit{Proceedings of 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food \&{} Environmental Technologies}. Springer Singapore, 2016, s.~192-195. ISBN~978-981-287-817-5. Dostupné z: https://dx.doi.org/10.1007/978-981-287-817-5.

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