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@article{1337368,
author = {Hernangómez Herrero, Miriam and Klusáková, Ilona and Joukal, Marek and Hradilová Svíženská, Ivana and Guaza, Carmen and Dubový, Petr},
article_location = {London},
article_number = {43},
doi = {http://dx.doi.org/10.1186/s12974-016-0508-8},
keywords = {Rat neuropathic pain model; Sterile nerve constriction; Neuroinflammation; Activated glial cells; Cytokines; Modulation},
language = {eng},
issn = {1742-2094},
journal = {Journal of Neuroinflammation},
title = {CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model},
volume = {13},
year = {2016}
}
TY - JOUR
ID - 1337368
AU - Hernangómez Herrero, Miriam - Klusáková, Ilona - Joukal, Marek - Hradilová Svíženská, Ivana - Guaza, Carmen - Dubový, Petr
PY - 2016
TI - CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model
JF - Journal of Neuroinflammation
VL - 13
IS - 43
SP - 1-15
EP - 1-15
PB - Biomed Central LTD
SN - 17422094
KW - Rat neuropathic pain model
KW - Sterile nerve constriction
KW - Neuroinflammation
KW - Activated glial cells
KW - Cytokines
KW - Modulation
N2 - Background: Interaction of CD200 with its receptor CD200R has an immunoregulatory role and attenuates various types of neuroinflammatory diseases. Methods: Immunofluorescence staining, western blot analysis, and RT-PCR were used to investigate the modulatory effects of CD200 fusion protein (CD200Fc) on activation of microglia and astrocytes as well as synthesis of pro- (TNF, IL-1beta, IL-6) and anti-inflammatory (IL-4, IL-10) cytokines in the L4–L5 spinal cord segments in relation to behavioral signs of neuropathic pain after unilateral sterile chronic constriction injury (sCCI) of the sciatic nerve. Withdrawal thresholds for mechanical hypersensitivity and latencies for thermal hypersensitivity were measured in hind paws 1 day before operation; 1, 3, and 7 days after sCCI operation; and then 5 and 24 h after intrathecal application of artificial cerebrospinal fluid or CD200Fc. Results: Seven days from sCCI operation and 5 h from intrathecal application, CD200Fc reduced mechanical and thermal hypersensitivity when compared with control animals. Simultaneously, CD200Fc attenuated activation of glial cells and decreased proinflammatory and increased anti-inflammatory cytokine messenger RNA (mRNA) levels. Administration of CD200Fc also diminished elevation of CD200 and CD200R proteins as a concomitant reaction of the modulatory system to increased neuroinflammatory reactions after nerve injury. The anti-inflammatory effect of CD200Fc dropped at 24 h after intrathecal application. Conclusions: Intrathecal administration of the CD200R1 agonist CD200Fc induces very rapid suppression of neuroinflammatory reactions associated with glial activation and neuropathic pain development. This may constitute a promising and novel therapeutic approach for the treatment of neuropathic pain.
ER -
HERNANGÓMEZ HERRERO, Miriam, Ilona KLUSÁKOVÁ, Marek JOUKAL, Ivana HRADILOVÁ SVÍŽENSKÁ, Carmen GUAZA and Petr DUBOVÝ. CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model. \textit{Journal of Neuroinflammation}. London: Biomed Central LTD, 2016, vol.~13, No~43, p.~1-15. ISSN~1742-2094. Available from: https://dx.doi.org/10.1186/s12974-016-0508-8.
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