| JAKEŠOVÁ, Hana, Jana ŘEPKOVÁ, Jan NEDĚLNÍK, David HAMPEL, Jana DLUHOŠOVÁ, Martina SOLDÁNOVÁ a Martina OŠŤÁDALOVÁ. Selecting plants with increased total polyphenol oxidases in the genus Trifolium. Online. Czech Journal of Genetics and Plant Breeding. Praha: Czech Academy of Agricultural Sciences, 2015, roč. 51, č. 4, s. 155-161. ISSN 1212-1975. Dostupné z: https://dx.doi.org/10.17221/107/2015-CJGPB. [citováno 2024-04-24] |
Další formáty:
BibTeX
LaTeX
RIS
@article{1337380,
author = {Jakešová, Hana and Řepková, Jana and Nedělník, Jan and Hampel, David and Dluhošová, Jana and Soldánová, Martina and Ošťádalová, Martina},
article_location = {Praha},
article_number = {4},
doi = {http://dx.doi.org/10.17221/107/2015-CJGPB},
keywords = {expression analysis; polyphenolic enzyme; protein quality; Trifolium medium; Trifolium pratense},
language = {eng},
issn = {1212-1975},
journal = {Czech Journal of Genetics and Plant Breeding},
title = {Selecting plants with increased total polyphenol oxidases in the genus Trifolium},
volume = {51},
year = {2015}
}
TY - JOUR
ID - 1337380
AU - Jakešová, Hana - Řepková, Jana - Nedělník, Jan - Hampel, David - Dluhošová, Jana - Soldánová, Martina - Ošťádalová, Martina
PY - 2015
TI - Selecting plants with increased total polyphenol oxidases in the genus Trifolium
JF - Czech Journal of Genetics and Plant Breeding
VL - 51
IS - 4
SP - 155-161
EP - 155-161
PB - Czech Academy of Agricultural Sciences
SN - 12121975
KW - expression analysis
KW - polyphenolic enzyme
KW - protein quality
KW - Trifolium medium
KW - Trifolium pratense
N2 - One of the aims in red clover (Trifolium pratense) breeding is to increase the polyphenol oxidase (PPO) activity, which may effectively reduce protein breakdown in silage and when cattle are fed by fresh clover. We analysed total PPO activity spectrophotometrically and on the level of gene expression using real-time quantitative PCR in single plants derived from an interspecific T. pratense × T. medium hybrid. Experiments were performed for two years and evaluated according to the general linear model with three factors (family, year, and cut). The analysis revealed considerable variability in total PPO activity between individuals and between families. Four families and two individuals with significantly higher PPO activity were selected. Their PPO activity ranged from 3.411 to 3.547 mkatal/min/g and from 4.041 to 5.731 mkatal/min/g, respectively, in comparison with the control variety Amos (2.370 mkatal/min/g). The majority of PPO transcripts were expressed by the two genes PPO1/5 and PPO2. In some genotypes, the PPO5 gene was expressed. Quantitative PCR confirmed the highest activity of PPO genes in seven hybrid plants with higher DNA contents corresponding to 30 chromosomes with 815 013 copies per plant. Our results indicate the suitability of combining two methods for improved selection: initial expression analysis to assess the PPO transcript level indicating gene activity and subsequent enzymatic assay.
ER -
JAKEŠOVÁ, Hana, Jana ŘEPKOVÁ, Jan NEDĚLNÍK, David HAMPEL, Jana DLUHOŠOVÁ, Martina SOLDÁNOVÁ a Martina OŠŤÁDALOVÁ. Selecting plants with increased total polyphenol oxidases in the genus Trifolium. Online. \textit{Czech Journal of Genetics and Plant Breeding}. Praha: Czech Academy of Agricultural Sciences, 2015, roč.~51, č.~4, s.~155-161. ISSN~1212-1975. Dostupné z: https://dx.doi.org/10.17221/107/2015-CJGPB. [citováno 2024-04-24]
|
