Classification of strain CCM 4446T as Rhodococcus degradans sp. nov.

ŠVEC, Pavel, Jitka ČERNOHLÁVKOVÁ, Hans Jurgen BUSSE, Hana VOJTKOVÁ, Roman PANTŮČEK, Margo CNOCKAERT, Ivana MAŠLAŇOVÁ, Stanislava KRÁLOVÁ, Peter VANDAMME and Ivo SEDLÁČEK. Classification of strain CCM 4446T as Rhodococcus degradans sp. nov. International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology, 2015, vol. 65, December, p. 4381-4387. ISSN 1466-5026. Available from: https://dx.doi.org/10.1099/ijsem.0.000584.

Basic information

• Original name: Classification of strain CCM 4446T as Rhodococcus degradans sp. nov.
• Authors: ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Jitka ČERNOHLÁVKOVÁ (203 Czech Republic, belonging to the institution), Hans Jurgen BUSSE (40 Austria), Hana VOJTKOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Margo CNOCKAERT (56 Belgium), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution), Stanislava KRÁLOVÁ (703 Slovakia, belonging to the institution), Peter VANDAMME (56 Belgium) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution).
• Edition: International Journal of Systematic and Evolutionary Microbiology, Society for General Microbiology, 2015, 1466-5026.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.439
• RIV identification code: RIV/00216224:14310/15:00086923
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1099/ijsem.0.000584
• UT WoS: 000370460600014
• Keywords in English: Rhodococcus degradans sp. nov.; taxonomy; biodegradation
• Tags: AKR, rivok
• Tags: International impact, Reviewed

Abstract

Strain CCM 4446T, with notable biodegradation capabilities, was investigated in this study in order to elucidate its taxonomic position. Chemotaxonomic analyses of quinones, polar lipids, mycolic acids, polyamines and the diamino acid of the cell-wall peptidoglycan corresponded with characteristics of the genus Rhodococcus. Phylogenetic analysis, based on the 16S rRNA gene sequence, assigned strain CCM 4446T to the genus Rhodococcus and placed it in the Rhodococcus erythropolis 16S rRNA gene clade. Further analysis of catA and gyrB gene sequences, automated ribotyping with EcoRI restriction endonuclease, whole-cell protein profiling, DNA–DNA hybridization and extensive biotyping enabled differentiation of strain CCM 4446T from all phylogenetically closely related species, i.e., Rhodococcus baikonurensis, Rhodococcus qingshengii, Rhodococcus erythropolis and Rhodococcus globerulus. The results obtained show that the strain investigated represents a novel species within the genus Rhodococcus, for which the name Rhodococcus degradans sp. nov., is proposed. The type strain is CCM 4446T (LMG 28633T).

Links

• EE2.3.20.0183, research and development project: Name: Centrum experimentální biomedicíny