ŠPANOVÁ, Alena, Vladimír DRÁB, Kristýna TURKOVÁ, Miroslav ŠPANO, Radka BURDYCHOVÁ, Ondrej ŠEDO, Dagmar ŠRŮTKOVÁ, Vojtěch RADA and Bohuslav RITTICH. Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry. European Food Research and Technology. New York: Springer, 2015, vol. 241, No 6, p. 861-869. ISSN 1438-2377. Available from: https://dx.doi.org/10.1007/s00217-015-2511-1.
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Basic information
Original name Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry
Authors ŠPANOVÁ, Alena (203 Czech Republic), Vladimír DRÁB (203 Czech Republic), Kristýna TURKOVÁ (203 Czech Republic), Miroslav ŠPANO (203 Czech Republic), Radka BURDYCHOVÁ (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, guarantor, belonging to the institution), Dagmar ŠRŮTKOVÁ (203 Czech Republic), Vojtěch RADA (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic).
Edition European Food Research and Technology, New York, Springer, 2015, 1438-2377.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 21101 Food and beverages
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW Full Text
Impact factor Impact factor: 1.433
RIV identification code RIV/00216224:14740/15:00080488
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1007/s00217-015-2511-1
UT WoS 000361828100014
Keywords in English Lactobacillus; Identification; MALDI-TOF MS; In vitro tests; Probiotic characteristics
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 17/12/2019 11:21.
Abstract
In the present study, phenotypic and genotypic methods and MALDI-TOF mass spectrometry were applied for the species identification of 29 out of 30 strains of the genus Lactobacillus isolated from faecal samples of 30 breastfed infants. All strains were tested for growth in milk and/or reconstituted skimmed milk supplemented with 0.5 % yeast extract, production of lactic acid, bile salts and low pH tolerance, and for production of beta-glucuronidase. PCR screenings of tyrosine-decarboxylase and histidine-decarboxylase genes were also carried out. Survival of Lactobacillus cells at low pH and their bile tolerance were strain specific. Finally, seven strains of probiotic species were selected for possible use in the dairy industry and for further tests concerning their probiotic characteristics.
Links
CZ.1.05/1.1.00/02.0068, interní kód MUName: CEITEC - středoevropský technologický institut (Acronym: CEITEC)
Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
1G58097, research and development projectName: Molekulárně-genetické metody pro identifikaci probiotických bakterií lidského původu s vhodnými technologickými a hygienicko-zdravotními vlastnostmi pro výrobu funkčních potravin.
Investor: Ministry of Agriculture of the CR, Molecular-genetic methods for identification of probiotic bakteria with good technologic, hygienic, and health properties
2B06053, research and development projectName: Nové postupy při charakterizaci a identifikaci probiotických kmenů bakterií vhodných pro funkční potraviny.
Investor: Ministry of Education, Youth and Sports of the CR, New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods
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