J 2015

Acinetobacter albensis sp nov., isolated from natural soil and water ecosystems

KŘÍŽOVÁ, Lenka, Martina MAIXNEROVÁ, Ondrej ŠEDO and Alexandr NEMEC

Basic information

Original name

Acinetobacter albensis sp nov., isolated from natural soil and water ecosystems

Authors

KŘÍŽOVÁ, Lenka (203 Czech Republic), Martina MAIXNEROVÁ (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, guarantor, belonging to the institution) and Alexandr NEMEC (203 Czech Republic)

Edition

International Journal of Systematic and Evolutionary Microbiology, Berks (UK), Society for General Microbiology, 2015, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.439

RIV identification code

RIV/00216224:14740/15:00081635

Organization unit

Central European Institute of Technology

DOI

UT WoS

000369637800024

Keywords in English

16S rRNA gene; RpoB gene; GyrB gene; MALDI-ToF MS; Phenotype

Tags

Změněno: 5/4/2016 16:50, Mgr. Eva Špillingová

Abstract

V originále

We have studied the taxonomic position of a phenetically unique group of eight strains of the genus Acinetobacter which were isolated from soil and water samples collected in protected landscape areas in the Czech Republic. Each of the comparative sequence analyses of the 16S rRNA, gyrB and rpoB genes showed that the eight strains formed a cohesive and tight cluster (intracluster sequence identities of >= 99.9 %, >= 98.5 % and >= 97.7 %, respectively), which was clearly separated from all hitherto known species of the genus Acinetobacter (<= 98.6 %, <= 84.5 % and <= 89.3 %, respectively). Congruent with these findings were the results of comparative sequence analysis of three additional housekeeping genes (gltA, pyrG and recA). This genotypic distinctness was mirrored by the uniqueness of the combination of a number of independent phenotypic markers including the whole-cell spectra produced by matrix-assisted laser desorption ionization time-of-flight (MALDI-ToF) MS and physiological and metabolic features. The most useful phenotypic features to differentiate the eight strains from all known species of the genus Acinetobacter were the ability to assimilate tricarballylate and the inability to grow at 35 degrees C or to assimilate ethanol or L-histidine. We conclude that the eight strains represent a novel environmental species for which the name Acinetobacter albensis sp. nov. is proposed. The type strain is ANC 4874(T) (=CCUG 67281(T)=CCM 8611(T)).

Links

GA13-26693S, research and development project
Name: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.
Investor: Czech Science Foundation