RADASZKIEWICZ, Katarzyna Anna, Dominika SÝKOROVÁ, Pavel KARAS, Jana KUDOVÁ, Lukáš KOHÚT, Lucia BINÓ, Josef VEČEŘA, Jan VÍTEČEK, Lukáš KUBALA and Jiří PACHERNÍK. Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro. Review of Scientific Instruments. Melville (USA): American Institute of Physics, 2016, vol. 87, No 2, p. "nestrankovano", 7 pp. ISSN 0034-6748. Available from: https://dx.doi.org/10.1063/1.4941776. |
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@article{1338725, author = {Radaszkiewicz, Katarzyna Anna and Sýkorová, Dominika and Karas, Pavel and Kudová, Jana and Kohút, Lukáš and Binó, Lucia and Večeřa, Josef and Víteček, Jan and Kubala, Lukáš and Pacherník, Jiří}, article_location = {Melville (USA)}, article_number = {2}, doi = {http://dx.doi.org/10.1063/1.4941776}, keywords = {cardiomyocytes; digital video; beating}, language = {eng}, issn = {0034-6748}, journal = {Review of Scientific Instruments}, title = {Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro}, url = {http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/87/2/1.4941776.pdf?expires=1457558113&id=id&accname=guest&checksum=4E6E84A242B8B6216A78B92ACD4D6AE6}, volume = {87}, year = {2016} }
TY - JOUR ID - 1338725 AU - Radaszkiewicz, Katarzyna Anna - Sýkorová, Dominika - Karas, Pavel - Kudová, Jana - Kohút, Lukáš - Binó, Lucia - Večeřa, Josef - Víteček, Jan - Kubala, Lukáš - Pacherník, Jiří PY - 2016 TI - Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro JF - Review of Scientific Instruments VL - 87 IS - 2 SP - "nestrankovano" EP - "nestrankovano" PB - American Institute of Physics SN - 00346748 KW - cardiomyocytes KW - digital video KW - beating UR - http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/87/2/1.4941776.pdf?expires=1457558113&id=id&accname=guest&checksum=4E6E84A242B8B6216A78B92ACD4D6AE6 N2 - The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro ER -
RADASZKIEWICZ, Katarzyna Anna, Dominika SÝKOROVÁ, Pavel KARAS, Jana KUDOVÁ, Lukáš KOHÚT, Lucia BINÓ, Josef VEČEŘA, Jan VÍTEČEK, Lukáš KUBALA and Jiří PACHERNÍK. Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro. \textit{Review of Scientific Instruments}. Melville (USA): American Institute of Physics, 2016, vol.~87, No~2, p.~''nestrankovano'', 7 pp. ISSN~0034-6748. Available from: https://dx.doi.org/10.1063/1.4941776.
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