Simple non-invasive analysis of embryonic stem cell-derived
cardiomyocytes beating in vitro

J 2016

Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

RADASZKIEWICZ, Katarzyna Anna, Dominika SÝKOROVÁ, Pavel KARAS, Jana KUDOVÁ, Lukáš KOHÚT et. al.

Basic information

Original name

Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

Authors

RADASZKIEWICZ, Katarzyna Anna (616 Poland, belonging to the institution), Dominika SÝKOROVÁ (203 Czech Republic, belonging to the institution), Pavel KARAS (203 Czech Republic, belonging to the institution), Jana KUDOVÁ (203 Czech Republic, belonging to the institution), Lukáš KOHÚT (203 Czech Republic, belonging to the institution), Lucia BINÓ (703 Slovakia, belonging to the institution), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Jan VÍTEČEK (203 Czech Republic), Lukáš KUBALA (203 Czech Republic, belonging to the institution) and Jiří PACHERNÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Review of Scientific Instruments, Melville (USA), American Institute of Physics, 2016, 0034-6748

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.515

RIV identification code

RIV/00216224:14310/16:00089517

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1063/1.4941776

UT WoS

000371740900257

Keywords (in Czech)

kardiomyocyty; digitalni video; tepani

Keywords in English

cardiomyocytes; digital video; beating

Abstract

V originále

The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro

Citovat

RADASZKIEWICZ, Katarzyna Anna, Dominika SÝKOROVÁ, Pavel KARAS, Jana KUDOVÁ, Lukáš KOHÚT, Lucia BINÓ, Josef VEČEŘA, Jan VÍTEČEK, Lukáš KUBALA and Jiří PACHERNÍK. Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro. Review of Scientific Instruments. Melville (USA): American Institute of Physics, 2016, vol. 87, No 2, p. "nestrankovano", 7 pp. ISSN 0034-6748. Available from: https://dx.doi.org/10.1063/1.4941776.

@article{1338725,
   author = {Radaszkiewicz, Katarzyna Anna and Sýkorová, Dominika and Karas, Pavel and Kudová, Jana and Kohút, Lukáš and Binó, Lucia and Večeřa, Josef and Víteček, Jan and Kubala, Lukáš and Pacherník, Jiří},
   article_location = {Melville (USA)},
   article_number = {2},
   doi = {http://dx.doi.org/10.1063/1.4941776},
   keywords = {cardiomyocytes; digital video; beating},
   language = {eng},
   issn = {0034-6748},
   journal = {Review of Scientific Instruments},
   title = {Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro},
   url = {http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/87/2/1.4941776.pdf?expires=1457558113&id=id&accname=guest&checksum=4E6E84A242B8B6216A78B92ACD4D6AE6},
   volume = {87},
   year = {2016}
}

TY  - JOUR
ID  - 1338725
AU  - Radaszkiewicz, Katarzyna Anna - Sýkorová, Dominika - Karas, Pavel - Kudová, Jana - Kohút, Lukáš - Binó, Lucia - Večeřa, Josef - Víteček, Jan - Kubala, Lukáš - Pacherník, Jiří
PY  - 2016
TI  - Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro
JF  - Review of Scientific Instruments
VL  - 87
IS  - 2
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - American Institute of Physics
SN  - 00346748
KW  - cardiomyocytes
KW  - digital video
KW  - beating
UR  - http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/87/2/1.4941776.pdf?expires=1457558113&id=id&accname=guest&checksum=4E6E84A242B8B6216A78B92ACD4D6AE6
N2  - The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro
ER  -

RADASZKIEWICZ, Katarzyna Anna, Dominika SÝKOROVÁ, Pavel KARAS, Jana KUDOVÁ, Lukáš KOHÚT, Lucia BINÓ, Josef VEČEŘA, Jan VÍTEČEK, Lukáš KUBALA and Jiří PACHERNÍK. Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro. \textit{Review of Scientific Instruments}. Melville (USA): American Institute of Physics, 2016, vol.~87, No~2, p.~''nestrankovano'', 7 pp. ISSN~0034-6748. Available from: https://dx.doi.org/10.1063/1.4941776.

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