Effect of arsenic and cyanobacterial co-exposure on
pathological, haematological and immunological parameters of
rainbow trout (Oncorhynchus mykiss)

J 2015

Effect of arsenic and cyanobacterial co-exposure on pathological, haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss)

PALÍKOVÁ, Miroslava, Ivana PAPEŽÍKOVÁ, Radovan KOPP, Jan MAREŠ, Zdena MARKOVÁ et. al.

Basic information

Original name

Effect of arsenic and cyanobacterial co-exposure on pathological, haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss)

Authors

PALÍKOVÁ, Miroslava (203 Czech Republic, guarantor), Ivana PAPEŽÍKOVÁ (203 Czech Republic), Radovan KOPP (203 Czech Republic), Jan MAREŠ (203 Czech Republic), Zdena MARKOVÁ (203 Czech Republic), Stanislav NAVRÁTIL (203 Czech Republic), Ondřej ADAMOVSKÝ (203 Czech Republic, belonging to the institution), Jiří KOHOUTEK (203 Czech Republic, belonging to the institution), Lukáš NAVRÁTIL (203 Czech Republic) and Luděk BLÁHA (203 Czech Republic, belonging to the institution)

Edition

Neuroendocrinology Letters, STOCKHOLM (SWEDEN), MAGHIRA & MAAS PUBLICATIONS, 2015, 0172-780X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40103 Fishery

Country of publisher

Sweden

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.946

RIV identification code

RIV/00216224:14310/15:00086973

Organization unit

Faculty of Science

UT WoS

000369404400008

Keywords in English

microcystin; multiple exposure; lymphocytosis; neutropenia; phagocytic activity; fish

Abstract

V originále

OBJECTIVES: Under environmental conditions, fish are simultaneously exposed to multiple stressors. This study provides new knowledge on the effects of controlled exposure to multiple stressors, namely cyanobacterial biomass and food contaminated with arsenic. METHODS: Rainbow trout were divided into six groups of 25 fish and exposed to different contaminant combinations for 30 days: 1) control group, 2) cyanobacterial biomass, 3 & 4) two groups exposed to arsenic at concentrations of 5 mg.kg(-1) and 50 mg.kg(-1) fish feed, and 5 & 6) two groups exposed to cyanobacterial biomass and arsenic combined. We then evaluated pathological, haematological and immunological parameters at 10, 20 and 30 days after exposure. RESULTS: Marked gross pathological findings were present in groups exposed to arsenic and arsenic/cyanobacteria after 30 days. A strong decrease in haemoglobin concentration was observed in all experimental groups receiving arsenic after 10 days exposure. Total leukocyte count increased markedly in fish exposed to cyanobacterial biomass, and to higher arsenic concentrations by the end of the experiment. Neutrophils decreased significantly at the end of exposure. Similarly, exposure to cyanobacteria and/or arsenic led to suppression of opsonised zymosan particle-induced neutrophil respiratory bursts. CONCLUSIONS: Our results demonstrate that the effects of exposure to toxic cyanobacterial biomass and arsenic on fish are enhanced when the contaminants are combined. In particular, long-term exposure led to disturbances in the white blood-cell count. Modulation of phagocytosis, which is the first line of defence against invading pathogens, suggests that the combined action leads to a decreased ability to control infection.

Links

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PALÍKOVÁ, Miroslava, Ivana PAPEŽÍKOVÁ, Radovan KOPP, Jan MAREŠ, Zdena MARKOVÁ, Stanislav NAVRÁTIL, Ondřej ADAMOVSKÝ, Jiří KOHOUTEK, Lukáš NAVRÁTIL and Luděk BLÁHA. Effect of arsenic and cyanobacterial co-exposure on pathological, haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss). Neuroendocrinology Letters. STOCKHOLM (SWEDEN): MAGHIRA & MAAS PUBLICATIONS, 2015, vol. 36, Suppl.1, p. 57-63. ISSN 0172-780X.

@article{1338883,
   author = {Palíková, Miroslava and Papežíková, Ivana and Kopp, Radovan and Mareš, Jan and Marková, Zdena and Navrátil, Stanislav and Adamovský, Ondřej and Kohoutek, Jiří and Navrátil, Lukáš and Bláha, Luděk},
   article_location = {STOCKHOLM (SWEDEN)},
   article_number = {Suppl.1},
   keywords = {microcystin; multiple exposure; lymphocytosis; neutropenia; phagocytic activity; fish},
   language = {eng},
   issn = {0172-780X},
   journal = {Neuroendocrinology Letters},
   note = {Opraven obor publikace},
   title = {Effect of arsenic and cyanobacterial co-exposure on pathological, haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss)},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/26757114},
   volume = {36},
   year = {2015}
}

TY  - JOUR
ID  - 1338883
AU  - Palíková, Miroslava - Papežíková, Ivana - Kopp, Radovan - Mareš, Jan - Marková, Zdena - Navrátil, Stanislav - Adamovský, Ondřej - Kohoutek, Jiří - Navrátil, Lukáš - Bláha, Luděk
PY  - 2015
TI  - Effect of arsenic and cyanobacterial co-exposure on pathological, haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss)
JF  - Neuroendocrinology Letters
VL  - 36
IS  - Suppl.1
SP  - 57-63
EP  - 57-63
PB  - MAGHIRA & MAAS PUBLICATIONS
SN  - 0172780X
N1  - Opraven obor publikace
KW  - microcystin
KW  - multiple exposure
KW  - lymphocytosis
KW  - neutropenia
KW  - phagocytic activity
KW  - fish
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26757114
L2  - http://www.ncbi.nlm.nih.gov/pubmed/26757114
N2  - OBJECTIVES: Under environmental conditions, fish are simultaneously exposed to multiple stressors. This study provides new knowledge on the effects of controlled exposure to multiple stressors, namely cyanobacterial biomass and food contaminated with arsenic. METHODS: Rainbow trout were divided into six groups of 25 fish and exposed to different contaminant combinations for 30 days: 1) control group, 2) cyanobacterial biomass, 3 & 4) two groups exposed to arsenic at concentrations of 5 mg.kg(-1) and 50 mg.kg(-1) fish feed, and 5 & 6) two groups exposed to cyanobacterial biomass and arsenic combined. We then evaluated pathological, haematological and immunological parameters at 10, 20 and 30 days after exposure. RESULTS: Marked gross pathological findings were present in groups exposed to arsenic and arsenic/cyanobacteria after 30 days. A strong decrease in haemoglobin concentration was observed in all experimental groups receiving arsenic after 10 days exposure. Total leukocyte count increased markedly in fish exposed to cyanobacterial biomass, and to higher arsenic concentrations by the end of the experiment. Neutrophils decreased significantly at the end of exposure. Similarly, exposure to cyanobacteria and/or arsenic led to suppression of opsonised zymosan particle-induced neutrophil respiratory bursts. CONCLUSIONS: Our results demonstrate that the effects of exposure to toxic cyanobacterial biomass and arsenic on fish are enhanced when the contaminants are combined. In particular, long-term exposure led to disturbances in the white blood-cell count. Modulation of phagocytosis, which is the first line of defence against invading pathogens, suggests that the combined action leads to a decreased ability to control infection.
ER  -

PALÍKOVÁ, Miroslava, Ivana PAPEŽÍKOVÁ, Radovan KOPP, Jan MAREŠ, Zdena MARKOVÁ, Stanislav NAVRÁTIL, Ondřej ADAMOVSKÝ, Jiří KOHOUTEK, Lukáš NAVRÁTIL and Luděk BLÁHA. Effect of arsenic and cyanobacterial co-exposure on pathological, haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss). \textit{Neuroendocrinology Letters}. STOCKHOLM (SWEDEN): MAGHIRA \&{} MAAS PUBLICATIONS, 2015, vol.~36, Suppl.1, p.~57-63. ISSN~0172-780X.

Detailed Information on Publication Record