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@article{1339488,
author = {Melicherová, Janka and Mazourová, Veronika and Valigurová, Andrea},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s00436-015-4841-0},
keywords = {cryptosporidia excystation oocyst sporozoite},
language = {eng},
issn = {0932-0113},
journal = {Parasitology Research},
title = {In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media},
url = {http://link.springer.com/article/10.1007/s00436-015-4841-0},
volume = {115},
year = {2016}
}
TY - JOUR
ID - 1339488
AU - Melicherová, Janka - Mazourová, Veronika - Valigurová, Andrea
PY - 2016
TI - In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media
JF - Parasitology Research
VL - 115
IS - 3
SP - 1113-1121
EP - 1113-1121
PB - Springer Berlin Heidelberg
SN - 09320113
KW - cryptosporidia excystation oocyst sporozoite
UR - http://link.springer.com/article/10.1007/s00436-015-4841-0
N2 - This study aimed to evaluate and document the excystation process of Cryptosporidium muris oocysts in various incubation media, and to monitor the behaviour of excysting and freshly excysted sporozoites. A test of oocyst viability, using fluorescent double staining with fluorescein diacetate and propidium iodide, was performed prior to each experimental assay. Light microscope observations confirmed that relatively often only three sporozoites were released; the fourth one either left the oocyst later together with a residual body or remained trapped within the oocyst wall. These results suggest that successful oocyst excystation is not limited by the viability of all four sporozoites. Darkening of oocysts to opaque and their specific movement (the so-called oocyst dancing) preceded the final excystation and liberation of sporozoites, while the dormant oocysts appeared refractive. The process of excystation in C. muris is not gradual as generally described in cryptosporidia but very rapid in an eruptive manner. Experiments were performed using oocysts stored at 4 °C for various time periods, as well as oocysts freshly shed from host rodents (Mastomys coucha) of different ages. The most suitable medium supporting high excystation rate (76 %) and prolonged motility of sporozoites was RPMI 1640, enriched with 5 % bovine serum albumin (BSA). Our results emphasize that to reliably evaluate the success of in vitro excystation of cryptosporidia, not only the number of released sporozoites in a set time period should be taken into consideration but also their subsequent activity (motility), as it is expected to be essential for the invasion of host cells.
ER -
MELICHEROVÁ, Janka, Veronika MAZOUROVÁ and Andrea VALIGUROVÁ. In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media. \textit{Parasitology Research}. Springer Berlin Heidelberg, vol.~115, No~3, p.~1113-1121. ISSN~0932-0113. doi:10.1007/s00436-015-4841-0. 2016.
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