In vitro excystation of Cryptosporidium muris oocysts and
viability of released sporozoites in different incubation media

J 2016

In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media

MELICHEROVÁ, Janka, Veronika MAZOUROVÁ and Andrea VALIGUROVÁ

Basic information

Original name

In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media

Authors

MELICHEROVÁ, Janka (703 Slovakia, belonging to the institution), Veronika MAZOUROVÁ (203 Czech Republic, belonging to the institution) and Andrea VALIGUROVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

Parasitology Research, Springer Berlin Heidelberg, 2016, 0932-0113

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.329

RIV identification code

RIV/00216224:14310/16:00087850

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00436-015-4841-0

UT WoS

000370868200021

Keywords (in Czech)

kryptosporidie excystace oocysta sporozoit

Keywords in English

cryptosporidia excystation oocyst sporozoite

Abstract

V originále

This study aimed to evaluate and document the excystation process of Cryptosporidium muris oocysts in various incubation media, and to monitor the behaviour of excysting and freshly excysted sporozoites. A test of oocyst viability, using fluorescent double staining with fluorescein diacetate and propidium iodide, was performed prior to each experimental assay. Light microscope observations confirmed that relatively often only three sporozoites were released; the fourth one either left the oocyst later together with a residual body or remained trapped within the oocyst wall. These results suggest that successful oocyst excystation is not limited by the viability of all four sporozoites. Darkening of oocysts to opaque and their specific movement (the so-called oocyst dancing) preceded the final excystation and liberation of sporozoites, while the dormant oocysts appeared refractive. The process of excystation in C. muris is not gradual as generally described in cryptosporidia but very rapid in an eruptive manner. Experiments were performed using oocysts stored at 4 °C for various time periods, as well as oocysts freshly shed from host rodents (Mastomys coucha) of different ages. The most suitable medium supporting high excystation rate (76 %) and prolonged motility of sporozoites was RPMI 1640, enriched with 5 % bovine serum albumin (BSA). Our results emphasize that to reliably evaluate the success of in vitro excystation of cryptosporidia, not only the number of released sporozoites in a set time period should be taken into consideration but also their subsequent activity (motility), as it is expected to be essential for the invasion of host cells.

Links

GAP505/11/1163, research and development project

Name: Protizánětlivá aktivita extraktů z rostlin Indonésie a jejich účinek na průběh oportunních parazitóz

Investor: Czech Science Foundation

7AMB14FR013, research and development project

Name: Mechanizmus buněčného pohybu výtrusovců (Apicomplexa) (Acronym: MOTAP)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MELICHEROVÁ, Janka, Veronika MAZOUROVÁ and Andrea VALIGUROVÁ. In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media. Parasitology Research. Springer Berlin Heidelberg, 2016, vol. 115, No 3, p. 1113-1121. ISSN 0932-0113. Available from: https://dx.doi.org/10.1007/s00436-015-4841-0.

@article{1339488,
   author = {Melicherová, Janka and Mazourová, Veronika and Valigurová, Andrea},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00436-015-4841-0},
   keywords = {cryptosporidia excystation oocyst sporozoite},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media},
   url = {http://link.springer.com/article/10.1007/s00436-015-4841-0},
   volume = {115},
   year = {2016}
}

TY  - JOUR
ID  - 1339488
AU  - Melicherová, Janka - Mazourová, Veronika - Valigurová, Andrea
PY  - 2016
TI  - In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media
JF  - Parasitology Research
VL  - 115
IS  - 3
SP  - 1113-1121
EP  - 1113-1121
PB  - Springer Berlin Heidelberg
SN  - 09320113
KW  - cryptosporidia excystation oocyst sporozoite
UR  - http://link.springer.com/article/10.1007/s00436-015-4841-0
N2  - This study aimed to evaluate and document the excystation process of Cryptosporidium muris oocysts in various incubation media, and to monitor the behaviour of excysting and freshly excysted sporozoites. A test of oocyst viability, using fluorescent double staining with fluorescein diacetate and propidium iodide, was performed prior to each experimental assay. Light microscope observations confirmed that relatively often only three sporozoites were released; the fourth one either left the oocyst later together with a residual body or remained trapped within the oocyst wall. These results suggest that successful oocyst excystation is not limited by the viability of all four sporozoites. Darkening of oocysts to opaque and their specific movement (the so-called oocyst dancing) preceded the final excystation and liberation of sporozoites, while the dormant oocysts appeared refractive. The process of excystation in C. muris is not gradual as generally described in cryptosporidia but very rapid in an eruptive manner. Experiments were performed using oocysts stored at 4 °C for various time periods, as well as oocysts freshly shed from host rodents (Mastomys coucha) of different ages. The most suitable medium supporting high excystation rate (76 %) and prolonged motility of sporozoites was RPMI 1640, enriched with 5 % bovine serum albumin (BSA). Our results emphasize that to reliably evaluate the success of in vitro excystation of cryptosporidia, not only the number of released sporozoites in a set time period should be taken into consideration but also their subsequent activity (motility), as it is expected to be essential for the invasion of host cells.
ER  -

MELICHEROVÁ, Janka, Veronika MAZOUROVÁ and Andrea VALIGUROVÁ. In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media. \textit{Parasitology Research}. Springer Berlin Heidelberg, 2016, vol.~115, No~3, p.~1113-1121. ISSN~0932-0113. Available from: https://dx.doi.org/10.1007/s00436-015-4841-0.

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