Single nucleotide polymorphisms in the Trx2/TXNIP and TrxR2
genes of the mitochondrial thioredoxin antioxidant system and
the risk of diabetic retinopathy in patients with Type 2
diabetes mellitus

J 2016

Single nucleotide polymorphisms in the Trx2/TXNIP and TrxR2 genes of the mitochondrial thioredoxin antioxidant system and the risk of diabetic retinopathy in patients with Type 2 diabetes mellitus

MANKOC RAMUS, Sara, Ines CILENSEK, Mojca GLOBOCNIK PETROVIC, Miroslav SOUČEK, Peter KRUŽLIAK et. al.

Základní údaje

Originální název

Single nucleotide polymorphisms in the Trx2/TXNIP and TrxR2 genes of the mitochondrial thioredoxin antioxidant system and the risk of diabetic retinopathy in patients with Type 2 diabetes mellitus

Autoři

MANKOC RAMUS, Sara (705 Slovinsko), Ines CILENSEK (705 Slovinsko), Mojca GLOBOCNIK PETROVIC (705 Slovinsko), Miroslav SOUČEK (203 Česká republika, domácí), Peter KRUŽLIAK (703 Slovensko, garant, domácí) a Daniel PETROVIC (705 Slovinsko)

Vydání

Journal of Diabetes and its Complications, New York, Elsevier Science, 2016, 1056-8727

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.734

Kód RIV

RIV/00216224:14110/16:00089548

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.jdiacomp.2015.11.021

UT WoS

000371195100003

Klíčová slova anglicky

Type 2 diabetes mellitus; Diabetic retinopathy; Oxidative stress; Thioredoxin antioxidant system; Single nucleotide polymorphism

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 8. 2016 15:46, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Background: Oxidative stress plays an important role in the pathogenesis of diabetes and its complications. The aim of this study was to examine the possible association between seven single nucleotide polymorphisms (SNPs) of the Trx2/TXNIP and TrxR2 genes encoding proteins involved in the thioredoxin antioxidant defence system and the risk of diabetic retinopthy (DR). Design: Cross-sectional case-control study. Participants: A total of 802 Slovenian patients with Type 2 diabetes mellitus; 277 patients with DR and 525 with no DR were enrolled. Methods: Patients genotypes of the SNPs; including rs8140110, rs7211, rs7212, rs4755, rs1548357, rs4485648 and rs5748469 were determined by the competitive allele specific PCR method. Main Outcome Measures: Each genotype of examined SNPs was regressed in a logistic model, assuming the co-dominant, dominant and the recessive models of inheritance with covariates of duration of diabetes, HbA1c, insulin therapy, total cholesterol and LDL cholesterol levels. Results: In the present study, for the first time we identified an association between the rs4485648 polymorphism of the TrxR2 gene and DR in Caucasians with Type 2 DM. The estimated ORs of adjusted logistic regression models were found to be as follows: 4.4 for CT heterozygotes, 4.3 for TT homozygotes (co-dominant genetic model) and 4.4 for CT + TT genotypes (dominant genetic model). Conclusions: In our case-control study we were not able to demonstrate any association between rs8140110, rs7211, rs7212, rs4755, rs1548357, and rs5748469 and DR, however, our findings provide evidence that the rs4485648 polymorphism of the TrxR2 gene might exert an independent effect on the development of DR.

Citovat

MANKOC RAMUS, Sara, Ines CILENSEK, Mojca GLOBOCNIK PETROVIC, Miroslav SOUČEK, Peter KRUŽLIAK a Daniel PETROVIC. Single nucleotide polymorphisms in the Trx2/TXNIP and TrxR2 genes of the mitochondrial thioredoxin antioxidant system and the risk of diabetic retinopathy in patients with Type 2 diabetes mellitus. Journal of Diabetes and its Complications. New York: Elsevier Science, 2016, roč. 30, č. 2, s. 192-198. ISSN 1056-8727. Dostupné z: https://dx.doi.org/10.1016/j.jdiacomp.2015.11.021.

@article{1339528,
   author = {Mankoc Ramus, Sara and Cilensek, Ines and Globocnik Petrovic, Mojca and Souček, Miroslav and Kružliak, Peter and Petrovic, Daniel},
   article_location = {New York},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.jdiacomp.2015.11.021},
   keywords = {Type 2 diabetes mellitus; Diabetic retinopathy; Oxidative stress; Thioredoxin antioxidant system; Single nucleotide polymorphism},
   language = {eng},
   issn = {1056-8727},
   journal = {Journal of Diabetes and its Complications},
   title = {Single nucleotide polymorphisms in the Trx2/TXNIP and TrxR2 genes of the mitochondrial thioredoxin antioxidant system and the risk of diabetic retinopathy in patients with Type 2 diabetes mellitus},
   volume = {30},
   year = {2016}
}

TY  - JOUR
ID  - 1339528
AU  - Mankoc Ramus, Sara - Cilensek, Ines - Globocnik Petrovic, Mojca - Souček, Miroslav - Kružliak, Peter - Petrovic, Daniel
PY  - 2016
TI  - Single nucleotide polymorphisms in the Trx2/TXNIP and TrxR2 genes of the mitochondrial thioredoxin antioxidant system and the risk of diabetic retinopathy in patients with Type 2 diabetes mellitus
JF  - Journal of Diabetes and its Complications
VL  - 30
IS  - 2
SP  - 192-198
EP  - 192-198
PB  - Elsevier Science
SN  - 10568727
KW  - Type 2 diabetes mellitus
KW  - Diabetic retinopathy
KW  - Oxidative stress
KW  - Thioredoxin antioxidant system
KW  - Single nucleotide polymorphism
N2  - Background: Oxidative stress plays an important role in the pathogenesis of diabetes and its complications. The aim of this study was to examine the possible association between seven single nucleotide polymorphisms (SNPs) of the Trx2/TXNIP and TrxR2 genes encoding proteins involved in the thioredoxin antioxidant defence system and the risk of diabetic retinopthy (DR). Design: Cross-sectional case-control study. Participants: A total of 802 Slovenian patients with Type 2 diabetes mellitus; 277 patients with DR and 525 with no DR were enrolled. Methods: Patients genotypes of the SNPs; including rs8140110, rs7211, rs7212, rs4755, rs1548357, rs4485648 and rs5748469 were determined by the competitive allele specific PCR method. Main Outcome Measures: Each genotype of examined SNPs was regressed in a logistic model, assuming the co-dominant, dominant and the recessive models of inheritance with covariates of duration of diabetes, HbA1c, insulin therapy, total cholesterol and LDL cholesterol levels. Results: In the present study, for the first time we identified an association between the rs4485648 polymorphism of the TrxR2 gene and DR in Caucasians with Type 2 DM. The estimated ORs of adjusted logistic regression models were found to be as follows: 4.4 for CT heterozygotes, 4.3 for TT homozygotes (co-dominant genetic model) and 4.4 for CT + TT genotypes (dominant genetic model). Conclusions: In our case-control study we were not able to demonstrate any association between rs8140110, rs7211, rs7212, rs4755, rs1548357, and rs5748469 and DR, however, our findings provide evidence that the rs4485648 polymorphism of the TrxR2 gene might exert an independent effect on the development of DR.
ER  -

MANKOC RAMUS, Sara, Ines CILENSEK, Mojca GLOBOCNIK PETROVIC, Miroslav SOUČEK, Peter KRUŽLIAK a Daniel PETROVIC. Single nucleotide polymorphisms in the Trx2/TXNIP and TrxR2 genes of the mitochondrial thioredoxin antioxidant system and the risk of diabetic retinopathy in patients with Type 2 diabetes mellitus. \textit{Journal of Diabetes and its Complications}. New York: Elsevier Science, 2016, roč.~30, č.~2, s.~192-198. ISSN~1056-8727. Dostupné z: https://dx.doi.org/10.1016/j.jdiacomp.2015.11.021.

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