| MOUKOVÁ, Lucie, Vladimíra VALLOVÁ, Iva SLÁMOVÁ, Miroslava KIŠŠOVÁ and Petr KUGLÍK. Initial experience with determination of hTERC and MYCC genes amplification in cervical intraepithelial neoplasia and cervical carcinoma in the Czech Republic. European Oncology and Haematology. Touch Briefing, London, 2012, vol. 8, No 2, p. 92-96. ISSN 1758-3853. |
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@article{1339572,
author = {Mouková, Lucie and Vallová, Vladimíra and Slámová, Iva and Kiššová, Miroslava and Kuglík, Petr},
article_number = {2},
keywords = {karcinom děložmího hrdla; cervikální intraepiteliální neoplázie; amplifikace genu hTERC; fluorescenční hybridizace in situ; genetické abnormality},
language = {eng},
issn = {1758-3853},
journal = {European Oncology and Haematology},
title = {Initial experience with determination of hTERC and MYCC genes amplification in cervical intraepithelial neoplasia and cervical carcinoma in the Czech Republic},
volume = {8},
year = {2012}
}
TY - JOUR
ID - 1339572
AU - Mouková, Lucie - Vallová, Vladimíra - Slámová, Iva - Kiššová, Miroslava - Kuglík, Petr
PY - 2012
TI - Initial experience with determination of hTERC and MYCC genes amplification in cervical intraepithelial neoplasia and cervical carcinoma in the Czech Republic
JF - European Oncology and Haematology
VL - 8
IS - 2
SP - 92-96
EP - 92-96
PB - Touch Briefing, London
SN - 17583853
KW - karcinom děložmího hrdla
KW - cervikální intraepiteliální neoplázie
KW - amplifikace genu hTERC
KW - fluorescenční hybridizace in situ
KW - genetické abnormality
N2 - Tumors are frequently characterized by series of cytogenetic abnormalities. Amplifications of hTERC (3q26) and MYCC (8q24) genes have been associated with cervical intraepithelial neoplasia (CIN) and carcinoma of uterine cervix. The results of genetic analysis could select patients with high risk of progression from CIN to carcinoma. In this study, chromosomal abnormalities in cytology specimens of cervical carcinoma or CIN of 26 patients were analyzed using new developed triple-colour HPV-FISH assay. HPV infection was proven in 85 % (22/26) of patients. Amplification of hTERC and MYCC gene was found in 46 % (12/26) and 62 % (16/26) of patients, respectively. Based on these results we were able to divide patients into high-risk, medium-risk and low-risk group. We confirmed that HPV-FISH assay can be used as an effective diagnostic procedure to identify patients carrying highly risking HPV infection and chromosomal aberrations associated with this malignancy. Patients of the high-risk group would benefit from intensive dispensarisation and aggressive therapy in the future. Tumors are frequently characterized by series of cytogenetic abnormalities. Amplifications of hTERC (3q26) and MYCC (8q24) genes have been associated with cervical intraepithelial neoplasia (CIN) and carcinoma of uterine cervix. The results of genetic analysis could select patients with high risk of progression from CIN to carcinoma. In this study, chromosomal abnormalities in cytology specimens of cervical carcinoma or CIN of 26 patients were analyzed using new developed triple-colour HPV-FISH assay. HPV infection was proven in 85 % (22/26) of patients. Amplification of hTERC and MYCC gene was found in 46 % (12/26) and 62 % (16/26) of patients, respectively. Based on these results we were able to divide patients into high-risk, medium-risk and low-risk group. We confirmed that HPV-FISH assay can be used as an effective diagnostic procedure to identify patients carrying highly risking HPV infection and chromosomal aberrations associated with this malignancy. Patients of the high-risk group would benefit from intensive dispensarisation and aggressive therapy in the future.
ER -
MOUKOVÁ, Lucie, Vladimíra VALLOVÁ, Iva SLÁMOVÁ, Miroslava KIŠŠOVÁ and Petr KUGLÍK. Initial experience with determination of hTERC and MYCC genes amplification in cervical intraepithelial neoplasia and cervical carcinoma in the Czech Republic. \textit{European Oncology and Haematology}. Touch Briefing, London, 2012, vol.~8, No~2, p.~92-96. ISSN~1758-3853.
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