Highly Sensitive Laser Scanning of Photon-Upconverting
Nanoparticles on a Macroscopic Scale

J 2016

Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale

SEDLMEIER, Andreas; Antonín HLAVÁČEK; Lucia BIRNER; Matthias MICKERT; Verena MUHR et. al.

Základní údaje

Originální název

Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale

Autoři

SEDLMEIER, Andreas (276 Německo); Antonín HLAVÁČEK (203 Česká republika, garant, domácí); Lucia BIRNER (276 Německo); Matthias MICKERT (276 Německo); Verena MUHR (276 Německo); Thomas HIRSCH (276 Německo); Paul CORSTJENS (528 Nizozemské království); Hans TANKE (528 Nizozemské království); Tero SOUKKA (246 Finsko) a Hans GORRIS (276 Německo)

Vydání

Analytical Chemistry, WASHINGTON, AMER CHEMICAL SOC, 2016, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.320

Kód RIV

RIV/00216224:14740/16:00089557

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1021/acs.analchem.5b04147

UT WoS

000369471100050

EID Scopus

2-s2.0-84957594448

Klíčová slova anglicky

CONVERTING PHOSPHOR REPORTERS; LANTHANIDE-DOPED NANOCRYSTALS; UP-CONVERSION NANOPARTICLES; CIRCULATING ANODIC ANTIGEN; LATERAL FLOW ASSAY; IMAGING IN-VIVO; FLUORESCENT; CELLS; NANOPHOSPHORS; LUMINESCENCE

Štítky

rivok

Změněno: 17. 3. 2017 13:40, Mgr. Eva Špillingová

Anotace

V originále

An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nano particles were detectable, and the linear dynamic range covered more than 5 orders of magnitude, which is essentially impossible by using conventional fluorescent dyes. UCNPs covered by a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventional fluorescence scanner as well as the upconversion scanner. Both optical labels could be detected independently. Finally, upconversion images of lateral flow test strips were recorded to facilitate the sensitive and quantitative detection of disease markers. A marker for the parasitic worm Schistosoma was used in this study.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

SEDLMEIER, Andreas; Antonín HLAVÁČEK; Lucia BIRNER; Matthias MICKERT; Verena MUHR; Thomas HIRSCH; Paul CORSTJENS; Hans TANKE; Tero SOUKKA a Hans GORRIS. Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale. Analytical Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2016, roč. 88, č. 3, s. 1835-1841. ISSN 0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.5b04147.

@article{1339695,
   author = {Sedlmeier, Andreas and Hlaváček, Antonín and Birner, Lucia and Mickert, Matthias and Muhr, Verena and Hirsch, Thomas and Corstjens, Paul and Tanke, Hans and Soukka, Tero and Gorris, Hans},
   article_location = {WASHINGTON},
   article_number = {3},
   doi = {https://doi.org/10.1021/acs.analchem.5b04147},
   keywords = {CONVERTING PHOSPHOR REPORTERS; LANTHANIDE-DOPED NANOCRYSTALS; UP-CONVERSION NANOPARTICLES; CIRCULATING ANODIC ANTIGEN; LATERAL FLOW ASSAY; IMAGING IN-VIVO; FLUORESCENT; CELLS; NANOPHOSPHORS; LUMINESCENCE},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale},
   url = {http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1339695
AU  - Sedlmeier, Andreas - Hlaváček, Antonín - Birner, Lucia - Mickert, Matthias - Muhr, Verena - Hirsch, Thomas - Corstjens, Paul - Tanke, Hans - Soukka, Tero - Gorris, Hans
PY  - 2016
TI  - Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale
JF  - Analytical Chemistry
VL  - 88
IS  - 3
SP  - 1835-1841
EP  - 1835-1841
PB  - AMER CHEMICAL SOC
SN  - 00032700
KW  - CONVERTING PHOSPHOR REPORTERS
KW  - LANTHANIDE-DOPED NANOCRYSTALS
KW  - UP-CONVERSION NANOPARTICLES
KW  - CIRCULATING ANODIC ANTIGEN
KW  - LATERAL FLOW ASSAY
KW  - IMAGING IN-VIVO
KW  - FLUORESCENT
KW  - CELLS
KW  - NANOPHOSPHORS
KW  - LUMINESCENCE
UR  - http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147
L2  - http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147
N2  - An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nano particles were detectable, and the linear dynamic range covered more than 5 orders of magnitude, which is essentially impossible by using conventional fluorescent dyes. UCNPs covered by a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventional fluorescence scanner as well as the upconversion scanner. Both optical labels could be detected independently. Finally, upconversion images of lateral flow test strips were recorded to facilitate the sensitive and quantitative detection of disease markers. A marker for the parasitic worm Schistosoma was used in this study.
ER  -

SEDLMEIER, Andreas; Antonín HLAVÁČEK; Lucia BIRNER; Matthias MICKERT; Verena MUHR; Thomas HIRSCH; Paul CORSTJENS; Hans TANKE; Tero SOUKKA a Hans GORRIS. Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale. \textit{Analytical Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2016, roč.~88, č.~3, s.~1835-1841. ISSN~0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.5b04147.

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