Highly Sensitive Laser Scanning of Photon-Upconverting
Nanoparticles on a Macroscopic Scale

J 2016

Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale

SEDLMEIER, Andreas, Antonín HLAVÁČEK, Lucia BIRNER, Matthias MICKERT, Verena MUHR et. al.

Basic information

Original name

Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale

Authors

SEDLMEIER, Andreas (276 Germany), Antonín HLAVÁČEK (203 Czech Republic, guarantor, belonging to the institution), Lucia BIRNER (276 Germany), Matthias MICKERT (276 Germany), Verena MUHR (276 Germany), Thomas HIRSCH (276 Germany), Paul CORSTJENS (528 Netherlands), Hans TANKE (528 Netherlands), Tero SOUKKA (246 Finland) and Hans GORRIS (276 Germany)

Edition

Analytical Chemistry, WASHINGTON, AMER CHEMICAL SOC, 2016, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.320

RIV identification code

RIV/00216224:14740/16:00089557

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/acs.analchem.5b04147

UT WoS

000369471100050

Keywords in English

CONVERTING PHOSPHOR REPORTERS; LANTHANIDE-DOPED NANOCRYSTALS; UP-CONVERSION NANOPARTICLES; CIRCULATING ANODIC ANTIGEN; LATERAL FLOW ASSAY; IMAGING IN-VIVO; FLUORESCENT; CELLS; NANOPHOSPHORS; LUMINESCENCE

Abstract

V originále

An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nano particles were detectable, and the linear dynamic range covered more than 5 orders of magnitude, which is essentially impossible by using conventional fluorescent dyes. UCNPs covered by a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventional fluorescence scanner as well as the upconversion scanner. Both optical labels could be detected independently. Finally, upconversion images of lateral flow test strips were recorded to facilitate the sensitive and quantitative detection of disease markers. A marker for the parasitic worm Schistosoma was used in this study.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

SEDLMEIER, Andreas, Antonín HLAVÁČEK, Lucia BIRNER, Matthias MICKERT, Verena MUHR, Thomas HIRSCH, Paul CORSTJENS, Hans TANKE, Tero SOUKKA and Hans GORRIS. Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale. Analytical Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2016, vol. 88, No 3, p. 1835-1841. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.5b04147.

@article{1339695,
   author = {Sedlmeier, Andreas and Hlaváček, Antonín and Birner, Lucia and Mickert, Matthias and Muhr, Verena and Hirsch, Thomas and Corstjens, Paul and Tanke, Hans and Soukka, Tero and Gorris, Hans},
   article_location = {WASHINGTON},
   article_number = {3},
   doi = {http://dx.doi.org/10.1021/acs.analchem.5b04147},
   keywords = {CONVERTING PHOSPHOR REPORTERS; LANTHANIDE-DOPED NANOCRYSTALS; UP-CONVERSION NANOPARTICLES; CIRCULATING ANODIC ANTIGEN; LATERAL FLOW ASSAY; IMAGING IN-VIVO; FLUORESCENT; CELLS; NANOPHOSPHORS; LUMINESCENCE},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale},
   url = {http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1339695
AU  - Sedlmeier, Andreas - Hlaváček, Antonín - Birner, Lucia - Mickert, Matthias - Muhr, Verena - Hirsch, Thomas - Corstjens, Paul - Tanke, Hans - Soukka, Tero - Gorris, Hans
PY  - 2016
TI  - Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale
JF  - Analytical Chemistry
VL  - 88
IS  - 3
SP  - 1835-1841
EP  - 1835-1841
PB  - AMER CHEMICAL SOC
SN  - 00032700
KW  - CONVERTING PHOSPHOR REPORTERS
KW  - LANTHANIDE-DOPED NANOCRYSTALS
KW  - UP-CONVERSION NANOPARTICLES
KW  - CIRCULATING ANODIC ANTIGEN
KW  - LATERAL FLOW ASSAY
KW  - IMAGING IN-VIVO
KW  - FLUORESCENT
KW  - CELLS
KW  - NANOPHOSPHORS
KW  - LUMINESCENCE
UR  - http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147
L2  - http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147
N2  - An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nano particles were detectable, and the linear dynamic range covered more than 5 orders of magnitude, which is essentially impossible by using conventional fluorescent dyes. UCNPs covered by a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventional fluorescence scanner as well as the upconversion scanner. Both optical labels could be detected independently. Finally, upconversion images of lateral flow test strips were recorded to facilitate the sensitive and quantitative detection of disease markers. A marker for the parasitic worm Schistosoma was used in this study.
ER  -

SEDLMEIER, Andreas, Antonín HLAVÁČEK, Lucia BIRNER, Matthias MICKERT, Verena MUHR, Thomas HIRSCH, Paul CORSTJENS, Hans TANKE, Tero SOUKKA and Hans GORRIS. Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale. \textit{Analytical Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2016, vol.~88, No~3, p.~1835-1841. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.5b04147.

Detailed Information on Publication Record