Detailed Information on Publication Record

ZLÁMALÍKOVÁ, Lenka, Mojmír MOULIS, David ŠÁLEK, Jiří JARKOVSKÝ, Jan ŠMARDA and Jana ŠMARDOVÁ. Expression of D-type cyclins in mantle cell and diffuse large B-cell lymphomas. Oncology Reports. Athens: Spandidos Publications, 2016, vol. 35, No 5, p. 2673-2680. ISSN 1021-335X. Available from: https://dx.doi.org/10.3892/or.2016.4658.

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Basic information
Original name	Expression of D-type cyclins in mantle cell and diffuse large B-cell lymphomas
Authors	ZLÁMALÍKOVÁ, Lenka (203 Czech Republic), Mojmír MOULIS (203 Czech Republic), David ŠÁLEK (203 Czech Republic), Jiří JARKOVSKÝ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Jana ŠMARDOVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition	Oncology Reports, Athens, Spandidos Publications, 2016, 1021-335X.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10601 Cell biology
Country of publisher	Greece
Confidentiality degree	is not subject to a state or trade secret
Impact factor	Impact factor: 2.662
RIV identification code	RIV/00216224:14310/16:00089581
Organization unit	Faculty of Science
Doi	http://dx.doi.org/10.3892/or.2016.4658
UT WoS	000374077700021
Keywords (in Czech)	lymfom mantlových buněk; difúzní lymfom velkých B-buněk; cykliny D; qRT-PCR; immunoblotting
Keywords in English	mantle cell lymphoma; diffuse large B- cell lymphoma; D-type cyclins; qRT-PCR; immunoblotting
Tags	AKR, podil
Tags	International impact, Reviewed
Changed by	Changed by: prof. RNDr. Jan Šmarda, CSc., učo 1223. Changed: 19/2/2018 11:36.

Abstract
D-type cyclins are involved in cell cycle regulation and play an important role in the pathogenesis of lymphomas. Aberrant expression of cyclin D1 is associated with mantle cell lymphoma (MCL) and serves as a diagnostic marker of MCL. Analysis of cyclin D expression in tumor tissues of patients with diffuse large B-cell lymphoma (DLBCL) which comprises a heterogeneous group of tumors may contribute to their stratification. We analyzed expression of cyclin D1, D2, and D3 mRNAs in 30 MCL and 104 DLBCL patients using qRT-PCR and addressed their significance for disease outcome. We confirmed a high level of cyclin D1 mRNA in 29 MCL cases (97%). One case (3%) was identified as positive for cyclin D2. Expression of cyclin D1 was limited to MCL and did not occur in DLBCL. Overexpression of cyclin D2, which is rare in MCL, occurred more frequently in DLBCL (11 cases, 10.6%). We showed that high expression of cyclin D2 in DLBCL cases de novo decreased the overall survival rate (P=0.016) and progression-free survival (P=0.009). The expression pattern of cyclin D3 was similar in both types of studied lymphomas and it did not affect the disease outcome.

Abstract

D-type cyclins are involved in cell cycle regulation and play an important role in the pathogenesis of lymphomas. Aberrant expression of cyclin D1 is associated with mantle cell lymphoma (MCL) and serves as a diagnostic marker of MCL. Analysis of cyclin D expression in tumor tissues of patients with diffuse large B-cell lymphoma (DLBCL) which comprises a heterogeneous group of tumors may contribute to their stratification. We analyzed expression of cyclin D1, D2, and D3 mRNAs in 30 MCL and 104 DLBCL patients using qRT-PCR and addressed their significance for disease outcome. We confirmed a high level of cyclin D1 mRNA in 29 MCL cases (97%). One case (3%) was identified as positive for cyclin D2. Expression of cyclin D1 was limited to MCL and did not occur in DLBCL. Overexpression of cyclin D2, which is rare in MCL, occurred more frequently in DLBCL (11 cases, 10.6%). We showed that high expression of cyclin D2 in DLBCL cases de novo decreased the overall survival rate (P=0.016) and progression-free survival (P=0.009). The expression pattern of cyclin D3 was similar in both types of studied lymphomas and it did not affect the disease outcome.