Detailed Information on Publication Record
2016
MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization
CELÁ, Andrea, Aleš MÁDR, Tereza DĚDOVÁ, Marta PELCOVÁ, Michal JEŠETA et. al.Basic information
Original name
MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization
Name in Czech
MEKC-LIF stanovení aminokyselin po on-capillary derivatizaci pomocí TDLFP pro stanovené životaschopnosti embryí pro účely asistované reprodukce pomocí kapilární elektroforézy s bezkontaktní vodivostní detekcí
Authors
CELÁ, Andrea (203 Czech Republic, belonging to the institution), Aleš MÁDR (203 Czech Republic, belonging to the institution), Tereza DĚDOVÁ (203 Czech Republic, belonging to the institution), Marta PELCOVÁ (203 Czech Republic, belonging to the institution), Michal JEŠETA (203 Czech Republic, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), Igor CRHA (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Electrophoresis, Wiley, 2016, 0173-0835
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.744
RIV identification code
RIV/00216224:14310/16:00087859
Organization unit
Faculty of Science
UT WoS
000383596800004
Keywords (in Czech)
asistovaná reprodukce; MEKC; LIF; aminokyseliny
Keywords in English
assisted reproduction; MEKC; LIF; amino acids
Tags
International impact, Reviewed
Změněno: 13/3/2018 10:53, Mgr. Andrea Celá, Ph.D.
V originále
Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo’s physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo’s surroundings can determine the embryo’s physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo’s surroundings was developed. AAs were analyzed by means of MEKC-LIF after on capillary derivatization by naphthalene 2,3 dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology. The resulting derivatives of all the standard AAs were baseline resolved in the background electrolyte comprised of 35 mM sodium tetraborate, 55 mM SDS, 2.7 M urea, 1 mM BIS-TRIS propane, and 23 mM NaOH within 50 minutes. The method was applied on an analysis of spent commercially available culture media used in assisted reproduction to culture embryos after in vitro fertilization.
In Czech
Význam asistované reprodukce roste vzhledem k rostoucímu počtu párů trpících neplodností. Klíčovými kroky asistované reprodukce jsou in vitro oplodnění vajíčka, kultivace embrya a výběr embrya s nejvyšším vývojovým potenciálem pro implantaci do dělohy. Současné stanovování vývojového potenciálu embrya je nepřesné, a proto jsou vyhledávány nové techniky k jeho lepšímu stanovení. Bylo vyvinutu MEKC-LIF stanovení aminokyselin po on-capillary derivatizaci pomocí TDLFP. Vyvinutá metoda byla úspěšně využita k analýze několika vzorků kultivačních médií odebraných po kultivaci embrya.
Links
| GAP206/11/0009, research and development project |
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| MUNI/A/1265/2015, interní kód MU |
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