| SCHULZ, Frederik, Tomáš TYML, Ilaria PIZZETTI, Iva DYKOVÁ, Stefano FAZI, Martin KOSTKA and Matthias HORN. Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2015, vol. 5, August, p. "nestránkováno", 10 pp. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/srep13381. |
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@article{1340509,
author = {Schulz, Frederik and Tyml, Tomáš and Pizzetti, Ilaria and Dyková, Iva and Fazi, Stefano and Kostka, Martin and Horn, Matthias},
article_location = {LONDON},
article_number = {August},
doi = {http://dx.doi.org/10.1038/srep13381},
keywords = {Microbial ecology; symbiosis},
language = {eng},
issn = {2045-2322},
journal = {Scientific Reports},
title = {Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria},
url = {http://www.nature.com/articles/srep13381},
volume = {5},
year = {2015}
}
TY - JOUR
ID - 1340509
AU - Schulz, Frederik - Tyml, Tomáš - Pizzetti, Ilaria - Dyková, Iva - Fazi, Stefano - Kostka, Martin - Horn, Matthias
PY - 2015
TI - Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria
JF - Scientific Reports
VL - 5
IS - August
SP - "nestránkováno"
EP - "nestránkováno"
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - Microbial ecology
KW - symbiosis
UR - http://www.nature.com/articles/srep13381
L2 - http://www.nature.com/articles/srep13381
N2 - Amoebae play an important ecological role as predators in microbial communities. They also serve as niche for bacterial replication, harbor endosymbiotic bacteria and have contributed to the evolution of major human pathogens. Despite their high diversity, marine amoebae and their association with bacteria are poorly understood. Here we describe the isolation and characterization of two novel marine amoebae together with their bacterial endosymbionts, tentatively named 'Candidatus Occultobacter vannellae' and 'Candidatus Nucleophilum amoebae'. While one amoeba strain is related to Vannella, a genus common in marine habitats, the other represents a novel lineage in the Amoebozoa. The endosymbionts showed only low similarity to known bacteria (85-88% 16S rRNA sequence similarity) but together with other uncultured marine bacteria form a sister clade to the Coxiellaceae. Using fluorescence in situ hybridization and transmission electron microscopy, identity and intracellular location of both symbionts were confirmed; one was replicating in host-derived vacuoles, whereas the other was located in the perinuclear space of its amoeba host. This study sheds for the first time light on a so far neglected group of protists and their bacterial symbionts. The newly isolated strains represent easily maintainable model systems and pave the way for further studies on marine associations between amoebae and bacterial symbionts.
ER -
SCHULZ, Frederik, Tomáš TYML, Ilaria PIZZETTI, Iva DYKOVÁ, Stefano FAZI, Martin KOSTKA and Matthias HORN. Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2015, vol.~5, August, p.~''nestránkováno'', 10 pp. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/srep13381.
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