| YOUNG, ND, Iva DYKOVÁ, PBB CROSBIE, M. WOLF, RN MORRISON, AR BRIDLE a BF NOWAK. Support for the coevolution of Neoparamoeba and their endosymbionts, Perkinsela amoebae-like organisms. European Journal of Protistology. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG, roč. 50, č. 5, s. 509-523. ISSN 0932-4739. doi:10.1016/j.ejop.2014.07.004. 2014. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1340737,
author = {Young, ND and Dyková, Iva and Crosbie, PBB and Wolf, M. and Morrison, RN and Bridle, AR and Nowak, BF},
article_location = {JENA},
article_number = {5},
doi = {http://dx.doi.org/10.1016/j.ejop.2014.07.004},
keywords = {Endosymbiont; ITS2; Neoparamoeba; Perkinsela; Phylogeny},
language = {eng},
issn = {0932-4739},
journal = {European Journal of Protistology},
title = {Support for the coevolution of Neoparamoeba and their endosymbionts, Perkinsela amoebae-like organisms},
url = {http://www.sciencedirect.com/science/article/pii/S0932473914000558},
volume = {50},
year = {2014}
}
TY - JOUR
ID - 1340737
AU - Young, ND - Dyková, Iva - Crosbie, PBB - Wolf, M. - Morrison, RN - Bridle, AR - Nowak, BF
PY - 2014
TI - Support for the coevolution of Neoparamoeba and their endosymbionts, Perkinsela amoebae-like organisms
JF - European Journal of Protistology
VL - 50
IS - 5
SP - 509-523
EP - 509-523
PB - ELSEVIER GMBH, URBAN & FISCHER VERLAG
SN - 09324739
KW - Endosymbiont
KW - ITS2
KW - Neoparamoeba
KW - Perkinsela
KW - Phylogeny
UR - http://www.sciencedirect.com/science/article/pii/S0932473914000558
L2 - http://www.sciencedirect.com/science/article/pii/S0932473914000558
N2 - Some of the species from the genus Neoparamoeba, for example N. perurans have been shown to be pathogenic to aquatic animals and thus have economic significance. They all contain endosymbiont, Perkinsela amoebae like organisms (PLOs). In this study we investigated phylogenetic ambiguities within the Neoparamoeba taxonomy and phylogenetic congruence between PLOs and their host Neoparamoeba to confirm the existence of a single ancient infection/colonisation that led to cospeciation between all PLOs and their host Neoparamoeba. DNA was extracted and rRNA genes from host amoeba and endosymbiont were amplified using PCR. Uncertainties in the Neoparamoeba phylogeny were initially resolved by a secondary phylogenetic marker, the internal transcribed spacer 2 (ITS2). The secondary structure of ITS2 was reconstructed for Neoparamoeba. The ITS2 was phylogenetically informative, separating N. pemaquidensis and N. aestuarina into distinct monophyletic clades and designating N. perurans as the most phylogenetically divergent Neoparamoeba species. The new phylogenetic data were used to verify the tree topologies used in cophylogenetic analyses that revealed strict phylogenetic congruence between endosymbiotic PLOs with their host Neoparamoeba. Strict congruence in the phylogeny of all PLOs and their host Neoparamoeba was demonstrated implying that PLOs are transmitted vertically from parent to daughter cell. (C) 2014 Elsevier GmbH. All rights reserved.
ER -
YOUNG, ND, Iva DYKOVÁ, PBB CROSBIE, M. WOLF, RN MORRISON, AR BRIDLE a BF NOWAK. Support for the coevolution of Neoparamoeba and their endosymbionts, Perkinsela amoebae-like organisms. \textit{European Journal of Protistology}. JENA: ELSEVIER GMBH, URBAN \&{}amp; FISCHER VERLAG, roč.~50, č.~5, s.~509-523. ISSN~0932-4739. doi:10.1016/j.ejop.2014.07.004. 2014.
|
