Dietary fatty acids specifically modulate phospholipid
patternin colon cells with distinct differentiation capacities

J 2017

Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities

HOFMANOVÁ, Jiřina, Josef SLAVÍK, Petra OVESNÁ, Zuzana TYLICHOVÁ, Jan VONDRÁČEK et. al.

Basic information

Original name

Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities

Authors

HOFMANOVÁ, Jiřina (203 Czech Republic), Josef SLAVÍK (203 Czech Republic), Petra OVESNÁ (203 Czech Republic, belonging to the institution), Zuzana TYLICHOVÁ (203 Czech Republic, belonging to the institution), Jan VONDRÁČEK (203 Czech Republic), Nicol STRAKOVÁ (203 Czech Republic), Alena HYRŠLOVÁ VACULOVÁ (203 Czech Republic), Miroslav CIGANEK (203 Czech Republic), Alois KOZUBÍK (203 Czech Republic, belonging to the institution), Lucia KNOPFOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Miroslav MACHALA (203 Czech Republic)

Edition

European Journal of Nutrition, Springer, 2017, 1436-6207

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.423

RIV identification code

RIV/00216224:14310/17:00095836

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00394-016-1196-y

UT WoS

000403469100011

Keywords (in Czech)

Apoptóza; butyrát; kardiolipiny; rakovina střeva; dokosahexanová kyselina; fosfolipidy

Keywords in English

Apoptosis; Butyrate; Cardiolipins; Colon cancer; Docosahexaenoic acid; Phospholipids

Abstract

V originále

Although beneficial effects of the dietary n-3 docosahexaenoic acid (DHA) or butyrate in colon carcinogenesis have been implicated, the mechanisms of their action are not fully clear. Here, we investigated modulations of composition of individual phospholipid (PL) classes, with a particular emphasis on cardiolipins (CLs), in colon cells treated with DHA, sodium butyrate (NaBt), or their combination (DHA/NaBt), and we evaluated possible associations between lipid changes and cell fate after fatty acid treatment. In two distinct human colon cell models, foetal colon (FHC) and adenocarcinoma (HCT-116) cells, we compared patterns and composition of individual PL classes following the fatty acid treatment by HPLC-MS/MS. In parallel, we measured the parameters reflecting cell proliferation, differentiation and death. In FHC cells, NaBt induced primarily differentiation, while co-treatment with DHA shifted their response towards cell death. In contrast, NaBt induced apoptosis in HCT-116 cells, which was not further affected by DHA. DHA was incorporated in all main PL types, increasing their unsaturation, while NaBt did not additionally modulate these effects in either cell model. Nevertheless, we identified an unusually wide range of CL species to be highly increased by NaBt and particularly by DHA/NaBt, and these effects were more pronounced in HCT-116 cells. DHA and DHA/NaBt enhanced levels of high molecular weight and more unsaturated CL species, containing DHA, which was specific for either differentiation or apoptotic responses. We identified a wide range of CL species in the colon cells which composition was significantly modified after DHA and NaBt treatment. These specific CL modulations might contribute to distinct cellular differentiation or apoptotic responses.

Citovat

HOFMANOVÁ, Jiřina, Josef SLAVÍK, Petra OVESNÁ, Zuzana TYLICHOVÁ, Jan VONDRÁČEK, Nicol STRAKOVÁ, Alena HYRŠLOVÁ VACULOVÁ, Miroslav CIGANEK, Alois KOZUBÍK, Lucia KNOPFOVÁ, Jan ŠMARDA and Miroslav MACHALA. Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities. European Journal of Nutrition. Springer, 2017, vol. 56, No 4, p. 1493-1508. ISSN 1436-6207. Available from: https://dx.doi.org/10.1007/s00394-016-1196-y.

@article{1340797,
   author = {Hofmanová, Jiřina and Slavík, Josef and Ovesná, Petra and Tylichová, Zuzana and Vondráček, Jan and Straková, Nicol and Hyršlová Vaculová, Alena and Ciganek, Miroslav and Kozubík, Alois and Knopfová, Lucia and Šmarda, Jan and Machala, Miroslav},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s00394-016-1196-y},
   keywords = {Apoptosis; Butyrate; Cardiolipins; Colon cancer; Docosahexaenoic acid; Phospholipids},
   language = {eng},
   issn = {1436-6207},
   journal = {European Journal of Nutrition},
   title = {Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities},
   volume = {56},
   year = {2017}
}

TY  - JOUR
ID  - 1340797
AU  - Hofmanová, Jiřina - Slavík, Josef - Ovesná, Petra - Tylichová, Zuzana - Vondráček, Jan - Straková, Nicol - Hyršlová Vaculová, Alena - Ciganek, Miroslav - Kozubík, Alois - Knopfová, Lucia - Šmarda, Jan - Machala, Miroslav
PY  - 2017
TI  - Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities
JF  - European Journal of Nutrition
VL  - 56
IS  - 4
SP  - 1493-1508
EP  - 1493-1508
PB  - Springer
SN  - 14366207
KW  - Apoptosis
KW  - Butyrate
KW  - Cardiolipins
KW  - Colon cancer
KW  - Docosahexaenoic acid
KW  - Phospholipids
N2  - Although beneficial effects of the dietary n-3 docosahexaenoic acid (DHA) or butyrate in colon carcinogenesis have been implicated, the mechanisms of their action are not fully clear. Here, we investigated modulations of composition of individual phospholipid (PL) classes, with a particular emphasis on cardiolipins (CLs), in colon cells treated with DHA, sodium butyrate (NaBt), or their combination (DHA/NaBt), and we evaluated possible associations between lipid changes and cell fate after fatty acid treatment. In two distinct human colon cell models, foetal colon (FHC) and adenocarcinoma (HCT-116) cells, we compared patterns and composition of individual PL classes following the fatty acid treatment by HPLC-MS/MS. In parallel, we measured the parameters reflecting cell proliferation, differentiation and death. In FHC cells, NaBt induced primarily differentiation, while co-treatment with DHA shifted their response towards cell death. In contrast, NaBt induced apoptosis in HCT-116 cells, which was not further affected by DHA. DHA was incorporated in all main PL types, increasing their unsaturation, while NaBt did not additionally modulate these effects in either cell model. Nevertheless, we identified an unusually wide range of CL species to be highly increased by NaBt and particularly by DHA/NaBt, and these effects were more pronounced in HCT-116 cells. DHA and DHA/NaBt enhanced levels of high molecular weight and more unsaturated CL species, containing DHA, which was specific for either differentiation or apoptotic responses. We identified a wide range of CL species in the colon cells which composition was significantly modified after DHA and NaBt treatment. These specific CL modulations might contribute to distinct cellular differentiation or apoptotic responses.
ER  -

HOFMANOVÁ, Jiřina, Josef SLAVÍK, Petra OVESNÁ, Zuzana TYLICHOVÁ, Jan VONDRÁČEK, Nicol STRAKOVÁ, Alena HYRŠLOVÁ VACULOVÁ, Miroslav CIGANEK, Alois KOZUBÍK, Lucia KNOPFOVÁ, Jan ŠMARDA and Miroslav MACHALA. Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities. \textit{European Journal of Nutrition}. Springer, 2017, vol.~56, No~4, p.~1493-1508. ISSN~1436-6207. Available from: https://dx.doi.org/10.1007/s00394-016-1196-y.

Detailed Information on Publication Record