HOFMANOVÁ, Jiřina, Josef SLAVÍK, Petra OVESNÁ, Zuzana TYLICHOVÁ, Jan VONDRÁČEK, Nicol STRAKOVÁ, Alena HYRŠLOVÁ VACULOVÁ, Miroslav CIGANEK, Alois KOZUBÍK, Lucia KNOPFOVÁ, Jan ŠMARDA and Miroslav MACHALA. Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities. European Journal of Nutrition. Springer, vol. 56, No 4, p. 1493-1508. ISSN 1436-6207. doi:10.1007/s00394-016-1196-y. 2017.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities
Authors HOFMANOVÁ, Jiřina (203 Czech Republic), Josef SLAVÍK (203 Czech Republic), Petra OVESNÁ (203 Czech Republic, belonging to the institution), Zuzana TYLICHOVÁ (203 Czech Republic, belonging to the institution), Jan VONDRÁČEK (203 Czech Republic), Nicol STRAKOVÁ (203 Czech Republic), Alena HYRŠLOVÁ VACULOVÁ (203 Czech Republic), Miroslav CIGANEK (203 Czech Republic), Alois KOZUBÍK (203 Czech Republic, belonging to the institution), Lucia KNOPFOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Miroslav MACHALA (203 Czech Republic).
Edition European Journal of Nutrition, Springer, 2017, 1436-6207.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.423
RIV identification code RIV/00216224:14310/17:00095836
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s00394-016-1196-y
UT WoS 000403469100011
Keywords (in Czech) Apoptóza; butyrát; kardiolipiny; rakovina střeva; dokosahexanová kyselina; fosfolipidy
Keywords in English Apoptosis; Butyrate; Cardiolipins; Colon cancer; Docosahexaenoic acid; Phospholipids
Tags AKR, NZ, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 21/2/2018 10:08.
Abstract
Although beneficial effects of the dietary n-3 docosahexaenoic acid (DHA) or butyrate in colon carcinogenesis have been implicated, the mechanisms of their action are not fully clear. Here, we investigated modulations of composition of individual phospholipid (PL) classes, with a particular emphasis on cardiolipins (CLs), in colon cells treated with DHA, sodium butyrate (NaBt), or their combination (DHA/NaBt), and we evaluated possible associations between lipid changes and cell fate after fatty acid treatment. In two distinct human colon cell models, foetal colon (FHC) and adenocarcinoma (HCT-116) cells, we compared patterns and composition of individual PL classes following the fatty acid treatment by HPLC-MS/MS. In parallel, we measured the parameters reflecting cell proliferation, differentiation and death. In FHC cells, NaBt induced primarily differentiation, while co-treatment with DHA shifted their response towards cell death. In contrast, NaBt induced apoptosis in HCT-116 cells, which was not further affected by DHA. DHA was incorporated in all main PL types, increasing their unsaturation, while NaBt did not additionally modulate these effects in either cell model. Nevertheless, we identified an unusually wide range of CL species to be highly increased by NaBt and particularly by DHA/NaBt, and these effects were more pronounced in HCT-116 cells. DHA and DHA/NaBt enhanced levels of high molecular weight and more unsaturated CL species, containing DHA, which was specific for either differentiation or apoptotic responses. We identified a wide range of CL species in the colon cells which composition was significantly modified after DHA and NaBt treatment. These specific CL modulations might contribute to distinct cellular differentiation or apoptotic responses.
PrintDisplayed: 28/3/2024 17:32