| ZÁBRADY, Kateřina, Marek ADAMUS, Lucie VONDROVÁ, Chunyan LIAO, Hana SKOUPILOVÁ, Markéta NOVÁKOVÁ, Lenka JURČIŠINOVÁ, Aaron ALT, Antony W. OLIVER, Alan R. LEHMANN and Jan PALEČEK. Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. Nucleic Acids Research. Oxford: Oxford University Press, 2016, vol. 44, No 3, p. 1064-1079. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkv1021. |
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@article{1340844,
author = {Zábrady, Kateřina and Adamus, Marek and Vondrová, Lucie and Liao, Chunyan and Skoupilová, Hana and Nováková, Markéta and Jurčišinová, Lenka and Alt, Aaron and Oliver, Antony W. and Lehmann, Alan R. and Paleček, Jan},
article_location = {Oxford},
article_number = {3},
doi = {http://dx.doi.org/10.1093/nar/gkv1021},
keywords = {SMC5-SMC6 COMPLEX; FISSION YEAST; SACCHAROMYCES-CEREVISIAE; CORE COMPONENT; REPAIR COMPLEX; ATP HYDROLYSIS; PROTEINS; COHESIN; REPLICATION; CHROMOSOMES},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA},
url = {https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021},
volume = {44},
year = {2016}
}
TY - JOUR
ID - 1340844
AU - Zábrady, Kateřina - Adamus, Marek - Vondrová, Lucie - Liao, Chunyan - Skoupilová, Hana - Nováková, Markéta - Jurčišinová, Lenka - Alt, Aaron - Oliver, Antony W. - Lehmann, Alan R. - Paleček, Jan
PY - 2016
TI - Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA
JF - Nucleic Acids Research
VL - 44
IS - 3
SP - 1064-1079
EP - 1064-1079
PB - Oxford University Press
SN - 03051048
KW - SMC5-SMC6 COMPLEX
KW - FISSION YEAST
KW - SACCHAROMYCES-CEREVISIAE
KW - CORE COMPONENT
KW - REPAIR COMPLEX
KW - ATP HYDROLYSIS
KW - PROTEINS
KW - COHESIN
KW - REPLICATION
KW - CHROMOSOMES
UR - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021
L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021
N2 - SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication fork stability and processing. However, the precise mechanism of action of SMC5/6 is not known. Here we present evidence that the NSE1/NSE3/NSE4 sub-complex of SMC5/6 binds to double-stranded DNA without any preference for DNA-replication/recombination intermediates. Mutations of key basic residues within the NSE1/NSE3/NSE4 DNA-binding surface reduce binding to DNA in vitro. Their introduction into the Schizosaccharomyces pombe genome results in cell death or hypersensitivity to DNA damaging agents. Chromatin immunoprecipitation analysis of the hypomorphic nse3 DNA-binding mutant shows a reduced association of fission yeast SMC5/6 with chromatin. Based on our results, we propose a model for loading of the SMC5/6 complex onto the chromatin.
ER -
ZÁBRADY, Kateřina, Marek ADAMUS, Lucie VONDROVÁ, Chunyan LIAO, Hana SKOUPILOVÁ, Markéta NOVÁKOVÁ, Lenka JURČIŠINOVÁ, Aaron ALT, Antony W. OLIVER, Alan R. LEHMANN and Jan PALEČEK. Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2016, vol.~44, No~3, p.~1064-1079. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkv1021.
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