Chromatin association of the SMC5/6 complex is dependent on
binding of its NSE3 subunit to DNA

J 2016

Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA

ZÁBRADY, Kateřina, Marek ADAMUS, Lucie VONDROVÁ, Chunyan LIAO, Hana SKOUPILOVÁ et. al.

Basic information

Original name

Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA

Authors

ZÁBRADY, Kateřina (203 Czech Republic, belonging to the institution), Marek ADAMUS (203 Czech Republic, belonging to the institution), Lucie VONDROVÁ (203 Czech Republic, belonging to the institution), Chunyan LIAO (826 United Kingdom of Great Britain and Northern Ireland), Hana SKOUPILOVÁ (203 Czech Republic, belonging to the institution), Markéta NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Lenka JURČIŠINOVÁ (703 Slovakia, belonging to the institution), Aaron ALT (826 United Kingdom of Great Britain and Northern Ireland), Antony W. OLIVER (826 United Kingdom of Great Britain and Northern Ireland), Alan R. LEHMANN (826 United Kingdom of Great Britain and Northern Ireland) and Jan PALEČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nucleic Acids Research, Oxford, Oxford University Press, 2016, 0305-1048

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.162

RIV identification code

RIV/00216224:14740/16:00087862

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/nar/gkv1021

UT WoS

000371268700017

Keywords in English

SMC5-SMC6 COMPLEX; FISSION YEAST; SACCHAROMYCES-CEREVISIAE; CORE COMPONENT; REPAIR COMPLEX; ATP HYDROLYSIS; PROTEINS; COHESIN; REPLICATION; CHROMOSOMES

Abstract

V originále

SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication fork stability and processing. However, the precise mechanism of action of SMC5/6 is not known. Here we present evidence that the NSE1/NSE3/NSE4 sub-complex of SMC5/6 binds to double-stranded DNA without any preference for DNA-replication/recombination intermediates. Mutations of key basic residues within the NSE1/NSE3/NSE4 DNA-binding surface reduce binding to DNA in vitro. Their introduction into the Schizosaccharomyces pombe genome results in cell death or hypersensitivity to DNA damaging agents. Chromatin immunoprecipitation analysis of the hypomorphic nse3 DNA-binding mutant shows a reduced association of fission yeast SMC5/6 with chromatin. Based on our results, we propose a model for loading of the SMC5/6 complex onto the chromatin.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0189, research and development project

Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

GA13-00774S, research and development project

Name: Úloha proteinů Nse, podjednotek komplexu SMC5/6, v procesech stabilizujících pozastavené replikační vidlice

Investor: Czech Science Foundation

Citovat

ZÁBRADY, Kateřina, Marek ADAMUS, Lucie VONDROVÁ, Chunyan LIAO, Hana SKOUPILOVÁ, Markéta NOVÁKOVÁ, Lenka JURČIŠINOVÁ, Aaron ALT, Antony W. OLIVER, Alan R. LEHMANN and Jan PALEČEK. Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. Nucleic Acids Research. Oxford: Oxford University Press, 2016, vol. 44, No 3, p. 1064-1079. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkv1021.

@article{1340844,
   author = {Zábrady, Kateřina and Adamus, Marek and Vondrová, Lucie and Liao, Chunyan and Skoupilová, Hana and Nováková, Markéta and Jurčišinová, Lenka and Alt, Aaron and Oliver, Antony W. and Lehmann, Alan R. and Paleček, Jan},
   article_location = {Oxford},
   article_number = {3},
   doi = {http://dx.doi.org/10.1093/nar/gkv1021},
   keywords = {SMC5-SMC6 COMPLEX; FISSION YEAST; SACCHAROMYCES-CEREVISIAE; CORE COMPONENT; REPAIR COMPLEX; ATP HYDROLYSIS; PROTEINS; COHESIN; REPLICATION; CHROMOSOMES},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA},
   url = {https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021},
   volume = {44},
   year = {2016}
}

TY  - JOUR
ID  - 1340844
AU  - Zábrady, Kateřina - Adamus, Marek - Vondrová, Lucie - Liao, Chunyan - Skoupilová, Hana - Nováková, Markéta - Jurčišinová, Lenka - Alt, Aaron - Oliver, Antony W. - Lehmann, Alan R. - Paleček, Jan
PY  - 2016
TI  - Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA
JF  - Nucleic Acids Research
VL  - 44
IS  - 3
SP  - 1064-1079
EP  - 1064-1079
PB  - Oxford University Press
SN  - 03051048
KW  - SMC5-SMC6 COMPLEX
KW  - FISSION YEAST
KW  - SACCHAROMYCES-CEREVISIAE
KW  - CORE COMPONENT
KW  - REPAIR COMPLEX
KW  - ATP HYDROLYSIS
KW  - PROTEINS
KW  - COHESIN
KW  - REPLICATION
KW  - CHROMOSOMES
UR  - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021
L2  - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021
N2  - SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication fork stability and processing. However, the precise mechanism of action of SMC5/6 is not known. Here we present evidence that the NSE1/NSE3/NSE4 sub-complex of SMC5/6 binds to double-stranded DNA without any preference for DNA-replication/recombination intermediates. Mutations of key basic residues within the NSE1/NSE3/NSE4 DNA-binding surface reduce binding to DNA in vitro. Their introduction into the Schizosaccharomyces pombe genome results in cell death or hypersensitivity to DNA damaging agents. Chromatin immunoprecipitation analysis of the hypomorphic nse3 DNA-binding mutant shows a reduced association of fission yeast SMC5/6 with chromatin. Based on our results, we propose a model for loading of the SMC5/6 complex onto the chromatin.
ER  -

ZÁBRADY, Kateřina, Marek ADAMUS, Lucie VONDROVÁ, Chunyan LIAO, Hana SKOUPILOVÁ, Markéta NOVÁKOVÁ, Lenka JURČIŠINOVÁ, Aaron ALT, Antony W. OLIVER, Alan R. LEHMANN and Jan PALEČEK. Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2016, vol.~44, No~3, p.~1064-1079. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkv1021.

Detailed Information on Publication Record