Other formats:
BibTeX
LaTeX
RIS
@article{1340931, author = {Legartová, Soňa and ŘezníčkováandPodloučková, Petra and Malyšková, Barbora and Küntziger, Thomas and Collas, Philippe and Cmarko, Dušan and Raška, Ivan and Sorokin, Dmitry and Kozubek, Stanislav and Bártová, Eva}, article_number = {11}, doi = {http://dx.doi.org/10.1002/jcb.25551}, keywords = {DNA REPAIR; 53BP1 PROTEIN; PML BODIES; HISTONE MODIFICATIONS; MICROSCOPY; PHOTOBLEACHING}, language = {eng}, issn = {0730-2312}, journal = {Journal of Cellular Biochemistry [IF=2.778 (1990)]}, title = {Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells}, volume = {117}, year = {2016} }
TY - JOUR ID - 1340931 AU - Legartová, Soňa - Řezníčková-Podloučková, Petra - Malyšková, Barbora - Küntziger, Thomas - Collas, Philippe - Cmarko, Dušan - Raška, Ivan - Sorokin, Dmitry - Kozubek, Stanislav - Bártová, Eva PY - 2016 TI - Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells JF - Journal of Cellular Biochemistry [IF=2.778 (1990)] VL - 117 IS - 11 SP - 2583-2596 EP - 2583-2596 PB - Wiley-Liss SN - 07302312 KW - DNA REPAIR KW - 53BP1 PROTEIN KW - PML BODIES KW - HISTONE MODIFICATIONS KW - MICROSCOPY KW - PHOTOBLEACHING N2 - We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and g-radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after g-irradiation. However, radiation did not change co-localization between 53BP1 nuclear bodies and interchromatin granule-associated zones (IGAZs), nuclear speckles, or chromocenters. This newly observed interaction pattern imply that 53BP1 protein could be a part of not only DNA repair, but also process mediated via components accumulated in IGAZs, nuclear speckles, or paraspeckles. Together, PML deficiency affected local motion of 53BP1 nuclear bodies and changed composition and a number of irradiation-induced foci. ER -
LEGARTOVÁ, Soňa, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Barbora MALYŠKOVÁ, Thomas KÜNTZIGER, Philippe COLLAS, Dušan CMARKO, Ivan RAŠKA, Dmitry SOROKIN, Stanislav KOZUBEK and Eva BÁRTOVÁ. Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells. \textit{Journal of Cellular Biochemistry [IF=2.778 (1990)]}. Wiley-Liss, 2016, vol.~117, No~11, p.~2583-2596. ISSN~0730-2312. Available from: https://dx.doi.org/10.1002/jcb.25551.
|