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@article{1340931,
author = {Legartová, Soňa and ŘezníčkováandPodloučková, Petra and Malyšková, Barbora and Küntziger, Thomas and Collas, Philippe and Cmarko, Dušan and Raška, Ivan and Sorokin, Dmitry and Kozubek, Stanislav and Bártová, Eva},
article_number = {11},
doi = {http://dx.doi.org/10.1002/jcb.25551},
keywords = {DNA REPAIR; 53BP1 PROTEIN; PML BODIES; HISTONE MODIFICATIONS; MICROSCOPY; PHOTOBLEACHING},
language = {eng},
issn = {0730-2312},
journal = {Journal of Cellular Biochemistry [IF=2.778 (1990)]},
title = {Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells},
volume = {117},
year = {2016}
}
TY - JOUR
ID - 1340931
AU - Legartová, Soňa - Řezníčková-Podloučková, Petra - Malyšková, Barbora - Küntziger, Thomas - Collas, Philippe - Cmarko, Dušan - Raška, Ivan - Sorokin, Dmitry - Kozubek, Stanislav - Bártová, Eva
PY - 2016
TI - Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells
JF - Journal of Cellular Biochemistry [IF=2.778 (1990)]
VL - 117
IS - 11
SP - 2583-2596
EP - 2583-2596
PB - Wiley-Liss
SN - 07302312
KW - DNA REPAIR
KW - 53BP1 PROTEIN
KW - PML BODIES
KW - HISTONE MODIFICATIONS
KW - MICROSCOPY
KW - PHOTOBLEACHING
N2 - We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and g-radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after g-irradiation. However, radiation did not change co-localization between 53BP1 nuclear bodies and interchromatin granule-associated zones (IGAZs), nuclear speckles, or chromocenters. This newly observed interaction pattern imply that 53BP1 protein could be a part of not only DNA repair, but also process mediated via components accumulated in IGAZs, nuclear speckles, or paraspeckles. Together, PML deficiency affected local motion of 53BP1 nuclear bodies and changed composition and a number of irradiation-induced foci.
ER -
LEGARTOVÁ, Soňa, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Barbora MALYŠKOVÁ, Thomas KÜNTZIGER, Philippe COLLAS, Dušan CMARKO, Ivan RAŠKA, Dmitry SOROKIN, Stanislav KOZUBEK and Eva BÁRTOVÁ. Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells. \textit{Journal of Cellular Biochemistry [IF=2.778 (1990)]}. Wiley-Liss, 2016, vol.~117, No~11, p.~2583-2596. ISSN~0730-2312. Available from: https://dx.doi.org/10.1002/jcb.25551.
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