RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF
VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE
MICROSCOPY TECHNIQUE

D 2015

RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE MICROSCOPY TECHNIQUE

THIRUPATHI, Karuppana Pandian, Christoph-Martin GEILFUS, Karl-Hermann MUHLING and Vít GLOSER

Basic information

Original name

RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE MICROSCOPY TECHNIQUE

Name in Czech

MĚŘENÍ ZMĚN PH APOPLASTU LISTŮ ROSTLIN VICIA FABA VYSTAVENÝM SUCHU POMOCÍ POMĚROVÉ FLUORESCENČNÍ METODY

Authors

THIRUPATHI, Karuppana Pandian, Christoph-Martin GEILFUS, Karl-Hermann MUHLING and Vít GLOSER

Edition

2015. vyd. Praha, Influence of abiotic and biotic stresses on properties of plants 2015, p. 137-140, 4 pp. 2015

Publisher

UEL - SAV Zvolen

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

Organization unit

Faculty of Science

ISBN

978-80-89408-23-8

Keywords in English

drought stress; leaf apoplastic pH; ratio imaging; Vicia faba

Abstract

V originále

We observed temporal dynamics of changes in apoplastic pH as a general stress response signal in fully developed leaves of Vicia faba (L.) plants both in diurnal cycle and during progressive soil drying. Use of H+-sensitive fluorescence probe in combination with ratio imaging microscopy allowed in situ measurements of intact plants with fine time resolution. Gradual increase of pH in apoplast started after 2 d of drought stress simultaneously with significantly decreased soil water content whereas changes in leaf water potential occurred later – after 4d of drought stress. The maximum change of 1.32 pH units was observed in leaf apoplast after 11 d of soil drying. The changes of pH were observed both in younger (top) leaves and in older (bottom) leaves but the amplitude was bigger in older leaves. The leaf apoplastic pH proved as a useful marker of stress signaling in plants.

Citovat

THIRUPATHI, Karuppana Pandian, Christoph-Martin GEILFUS, Karl-Hermann MUHLING and Vít GLOSER. RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE MICROSCOPY TECHNIQUE. In František Hnilička. Influence of abiotic and biotic stresses on properties of plants 2015. 2015th ed. Praha: UEL - SAV Zvolen, 2015, p. 137-140. ISBN 978-80-89408-23-8.

@inproceedings{1341003,
   author = {Thirupathi, Karuppana Pandian and Geilfus, ChristophandMartin and Muhling, KarlandHermann and Gloser, Vít},
   address = {Praha},
   booktitle = {Influence of abiotic and biotic stresses on properties of plants 2015},
   edition = {2015},
   editor = {František Hnilička},
   keywords = {drought stress; leaf apoplastic pH; ratio imaging; Vicia faba},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Praha},
   isbn = {978-80-89408-23-8},
   pages = {137-140},
   publisher = {UEL - SAV Zvolen},
   title = {RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE MICROSCOPY TECHNIQUE},
   year = {2015}
}

TY  - JOUR
ID  - 1341003
AU  - Thirupathi, Karuppana Pandian - Geilfus, Christoph-Martin - Muhling, Karl-Hermann - Gloser, Vít
PY  - 2015
TI  - RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE MICROSCOPY TECHNIQUE
PB  - UEL - SAV Zvolen
CY  - Praha
SN  - 9788089408238
KW  - drought stress
KW  - leaf apoplastic pH
KW  - ratio imaging
KW  - Vicia faba
N2  - We observed temporal dynamics of changes in apoplastic pH as a general stress response signal in fully developed leaves of Vicia faba (L.) plants both in diurnal cycle and during progressive soil drying. Use of H+-sensitive fluorescence probe in combination with ratio imaging microscopy allowed in situ measurements of intact plants with fine time resolution. Gradual increase of pH in apoplast started after 2 d of drought stress simultaneously with significantly decreased soil water content whereas changes in leaf water potential occurred later – after 4d of drought stress. The maximum change of 1.32 pH units was observed in leaf apoplast after 11 d of soil drying. The changes of pH were observed both in younger (top) leaves and in older (bottom) leaves but the amplitude was bigger in older leaves. The leaf apoplastic pH proved as a useful marker of stress signaling in plants.
ER  -

THIRUPATHI, Karuppana Pandian, Christoph-Martin GEILFUS, Karl-Hermann MUHLING and Vít GLOSER. RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE MICROSCOPY TECHNIQUE. In František Hnilička. \textit{Influence of abiotic and biotic stresses on properties of plants 2015}. 2015th ed. Praha: UEL - SAV Zvolen, 2015, p.~137-140. ISBN~978-80-89408-23-8.

Detailed Information on Publication Record