Detailed Information on Publication Record
2015
Hematopoietic developmental potential is variable in pluripotent stem cell lines
TESAŘOVÁ, Lenka, Barbara ŠALINGOVÁ, Pavel ŠIMARA and Irena KRONTORÁD KOUTNÁBasic information
Original name
Hematopoietic developmental potential is variable in pluripotent stem cell lines
Authors
TESAŘOVÁ, Lenka, Barbara ŠALINGOVÁ, Pavel ŠIMARA and Irena KRONTORÁD KOUTNÁ
Edition
Conference Frontiers in Material and Life Sciences: Creating Life in 3D, 2-4 September, 2015, Brno, 2015
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10601 Cell biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Informatics
Keywords (in Czech)
pluripotentní kmenové buňky; hematopoetický vývoj
Keywords in English
pluripotent stem cells; hematopoietic development
Změněno: 2/3/2018 09:59, Mgr. Pavel Šimara, Ph.D.
Abstract
V originále
The clinical application of hematopoietic precursors generated from human pluripotent stem cells (hPSCs) is still limited by low efficient differentiation protocols and functional defects in the derived cells. Moreover, the potential of various hPSCs to differentiate into blood cells has not been examined properly. In this study, protocols for hematopoietic differentiation were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines to determine their hematopoietic developmental potential. These protocols included different methods for hPSC cultivation and embryoid bodies (EBs) formation and composition of differentiation media. The efficiency of hematopoietic differentiation was found to be variable among hPSC lines. No protocol optimization enabled generation of CD34+ hematopoietic precursors from available hESC lines CCTL-12 and CCTL-14. On the contrary, these precursors were detected during hiPSC differentiation in basic media supplemented with three cytokines. The yield of precursors was variable, ranging from 0.8 to 10.3 percent of CD34+ cells and from 1.0 to 10.6 percent of CD43+ cells at day seven of differentiation. This yield was further increased when hiPSCs were differentiated for ten days in media with richer cytokine supplement. Nevertheless the variability among hiPSC lines was retained, 6.4 to 16.3 percent of cells were CD34+ and 7.8 to 20.0 percent of cells were CD43+235+ precursors of primitive hematopoiesis, while CD45+ precursors of definitive hematopoiesis comprised only from 0.4 to 4.7 percent. These results indicate that hematopoietic developmental potential of individual hPSC lines is characterised by significant variability that has to be overcome before blood cells derived from hPSC could be used for clinical applications.
Links
| CZ.1.07/2.3.00/30.0030, interní kód MU |
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| GBP302/12/G157, research and development project |
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