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@article{1341794,
author = {Veselý, Radek and Jelinkova, Pavlina and Hegerova, Dagmar and Cernei, Natalia and Kopel, Pavel and Moulick, Amitava and Richtera, Lukas and Heger, Zbynek and Adam, Vojtech and Zitka, Ondrej},
article_location = {Basel},
article_number = {4},
doi = {http://dx.doi.org/10.3390/ma9040260},
keywords = {branched chain amino acids; encapsulation; Escherichia coli; nanomedicine; Staphylococcus aureus},
language = {eng},
issn = {1996-1944},
journal = {Materials},
title = {Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides},
url = {http://www.mdpi.com/journal/materials},
volume = {9},
year = {2016}
}
TY - JOUR
ID - 1341794
AU - Veselý, Radek - Jelinkova, Pavlina - Hegerova, Dagmar - Cernei, Natalia - Kopel, Pavel - Moulick, Amitava - Richtera, Lukas - Heger, Zbynek - Adam, Vojtech - Zitka, Ondrej
PY - 2016
TI - Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides
JF - Materials
VL - 9
IS - 4
SP - 1-15
EP - 1-15
PB - MDPI AG
SN - 19961944
KW - branched chain amino acids
KW - encapsulation
KW - Escherichia coli
KW - nanomedicine
KW - Staphylococcus aureus
UR - http://www.mdpi.com/journal/materials
N2 - This paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)-leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome cavity resulting in a delivery system. Analyses of valine and leucine in entire complex show that 31.3% and 32.6% recoveries are reached for those amino acids. Evaluation of results shows that the success rate of delivery in Escherichia coli (E. coli) is higher in the case of BCAAs on nanoparticles entrapped in liposomes (28.7% and 34.7% for valine and leucine, respectively) when compared to nanoparticles with no liposomal envelope (18.3% and 13.7% for valine and leucine, respectively). The nanoparticles with no liposomal envelope exhibit the negative zeta potential (-9.1 +/- 0.3 mV); however, their encapsulation results in a shift into positive values (range of 28.9 +/- 0.4 to 33.1 +/- 0.5 mV). Thus, electrostatic interactions with negatively-charged cell membranes (approx. -50 mV in the case of E. coli) leads to a better uptake of cargo. Our delivery system was finally tested with the leucine-rich antimicrobial peptide (FALALKALKKALKKLKKALKKAL) and it is shown that hemocompatibility (7.5%) and antimicrobial activity of the entire complex against E. coli, Staphylococcus aureus (S. aureus), and methicilin-resistant S. aureus (MRSA) is comparable or better than conventional penicillin antibiotics.
ER -
VESELÝ, Radek, Pavlina JELINKOVA, Dagmar HEGEROVA, Natalia CERNEI, Pavel KOPEL, Amitava MOULICK, Lukas RICHTERA, Zbynek HEGER, Vojtech ADAM and Ondrej ZITKA. Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides. \textit{Materials}. Basel: MDPI AG, 2016, vol.~9, No~4, p.~1-15. ISSN~1996-1944. Available from: https://dx.doi.org/10.3390/ma9040260.
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