| NOSKOVÁ, Libuše, Božena KUBÍČKOVÁ, Lucie VAŠKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Marie STIBOROVÁ and Petr HODEK. Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence. Sensors. Basel: MDPI AG, 2015, vol. 15, No 1, p. 1945-1953. ISSN 1424-8220. Available from: https://dx.doi.org/10.3390/s150101945. |
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@article{1342171,
author = {Nosková, Libuše and Kubíčková, Božena and Vašková, Lucie and Bláhová, Barbora and Wimmerová, Michaela and Stiborová, Marie and Hodek, Petr},
article_location = {Basel},
article_number = {1},
doi = {http://dx.doi.org/10.3390/s150101945},
keywords = {cystic fibrosis; lungs; bacterial infection; lectin; chicken antibody; cell line; dual fluorescence},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327110/},
volume = {15},
year = {2015}
}
TY - JOUR
ID - 1342171
AU - Nosková, Libuše - Kubíčková, Božena - Vašková, Lucie - Bláhová, Barbora - Wimmerová, Michaela - Stiborová, Marie - Hodek, Petr
PY - 2015
TI - Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence
JF - Sensors
VL - 15
IS - 1
SP - 1945-1953
EP - 1945-1953
PB - MDPI AG
SN - 14248220
KW - cystic fibrosis
KW - lungs
KW - bacterial infection
KW - lectin
KW - chicken antibody
KW - cell line
KW - dual fluorescence
UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327110/
L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327110/
N2 - Antibodies against Pseudomonas aeruginosa (PA) lectin, PAIIL, which is a virulence factor mediating the bacteria binding to epithelium cells, were prepared in chickens and purified from egg yolks. To examine these antibodies as a prophylactic agent preventing the adhesion of PA we developed a well plate assay based on fluorescently labeled bacteria and immortalized epithelium cell lines derived from normal and cystic fibrosis (CF) human lungs. The antibodies significantly inhibited bacteria adhesion (up to 50%) in both cell lines. In agreement with in vivo data, our plate assay showed higher susceptibility of CF cells towards the PA adhesion as compared to normal epithelium. This finding proved the reliability of the developed experimental system.
ER -
NOSKOVÁ, Libuše, Božena KUBÍČKOVÁ, Lucie VAŠKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Marie STIBOROVÁ and Petr HODEK. Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence. \textit{Sensors}. Basel: MDPI AG, 2015, vol.~15, No~1, p.~1945-1953. ISSN~1424-8220. Available from: https://dx.doi.org/10.3390/s150101945.
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