Detailed Information on Publication Record
2016
Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)
SEDLÁČEK, Ivo, Eva STAŇKOVÁ and Pavel ŠVECBasic information
Original name
Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)
Authors
SEDLÁČEK, Ivo (203 Czech Republic, guarantor, belonging to the institution), Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution) and Pavel ŠVEC (203 Czech Republic, belonging to the institution)
Edition
Czech Journal of Animal Science, 2016, 1212-1819
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.741
RIV identification code
RIV/00216224:14310/16:00089679
Organization unit
Faculty of Science
UT WoS
000375410300004
Keywords in English
gut microflora; selective media; identification; Enterobacter; Aeromonas
Změněno: 13/3/2018 23:30, prof. RNDr. Ivo Sedláček, CSc.
Abstract
V originále
In this study, the intestinal bacteria of wild Antarctic fish (family Nototeniidae) were examined with traditional culture-based techniques. The bacterial flora of the gut contents of four marine fish species Notothenia coriiceps, Trematomus bernacchii, Trematomus hansoni and Trematomus newnesi has been investigated in an attempt to describe the allochthonous bacteria inhabiting these Nototheniidae fish. A set of 43 fresh and healthy fish was analysed and intestinal bacteria were retrieved using the dilution plate technique on selective media Endo agar and XLDA agar. A total of 133 different bacterial isolates were obtained and initially characterised by key phenotypical tests. Notothenoid fish gut microbiota showed low species diversity of isolates and intestines were inhabited in average amount of 3 different isolates per fish. The poor bacterial colonization of intestine content showed samples of Trematomus newnesi. Curiously, the Gram-negative non-fermenters, including Pseudomonas sp., Vibrio sp. and Alcaligenes-like isolates represented the majority (59%) of intestine isolates grown on the used selective media for enteric bacteria. Based on preliminary identification, only 54 isolates (41%) were tentatively determined as enteric bacteria. The identification of 54 isolates of Gram-negative fermenting rods to the species level was achieved using biochemical characterization by commercial kits ENTEROtest 24 and Biolog GN2 MicroPlate. Results showed that Enterobacter cloacae phenon and Aeromonas hydrophila were predominant bacterial species in the free-living fish intestine from the group of fermenting Gram-negative rods.
Links
| LM2010009, research and development project |
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