| SKAAR, Karin, Henryj J. KORZA, Michael TARRY, Petra SEKYROVÁ and Martin HOGBOM. Expression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiae. Plos one. San Francisco: Public Library of Science, 2015, vol. 10, No 7, p. nestránkováno, 19 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0134041. |
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@article{1342269,
author = {Skaar, Karin and Korza, Henryj J. and Tarry, Michael and Sekyrová, Petra and Hogbom, Martin},
article_location = {San Francisco},
article_number = {7},
doi = {http://dx.doi.org/10.1371/journal.pone.0134041},
keywords = {FAMILIAL EXUDATIVE VITREORETINOPATHY; EUKARYOTIC MEMBRANE-PROTEINS; ACUTE LYMPHOBLASTIC-LEUKEMIA; MALEIC ACID COPOLYMER; ENDOPLASMIC-RETICULUM; EXTRACELLULAR DOMAIN; FREE PURIFICATION; ESCHERICHIA-COLI; PICHIA-PASTORIS; QUALITY-CONTROL},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Expression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiae},
url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134041},
volume = {10},
year = {2015}
}
TY - JOUR
ID - 1342269
AU - Skaar, Karin - Korza, Henryj J. - Tarry, Michael - Sekyrová, Petra - Hogbom, Martin
PY - 2015
TI - Expression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiae
JF - Plos one
VL - 10
IS - 7
SP - nestránkováno
EP - nestránkováno
PB - Public Library of Science
SN - 19326203
KW - FAMILIAL EXUDATIVE VITREORETINOPATHY
KW - EUKARYOTIC MEMBRANE-PROTEINS
KW - ACUTE LYMPHOBLASTIC-LEUKEMIA
KW - MALEIC ACID COPOLYMER
KW - ENDOPLASMIC-RETICULUM
KW - EXTRACELLULAR DOMAIN
KW - FREE PURIFICATION
KW - ESCHERICHIA-COLI
KW - PICHIA-PASTORIS
KW - QUALITY-CONTROL
UR - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134041
L2 - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134041
N2 - Tetraspanins are integral membrane proteins that function as organizers of multimolecular complexes and modulate function of associated proteins. Mammalian genomes encode approximately 30 different members of this family and remotely related eukaryotic species also contain conserved tetraspanin homologs. Tetraspanins are involved in a number of fundamental processes such as regulation of cell migration, fusion, immunity and signaling. Moreover, they are implied in numerous pathological states including mental disorders, infectious diseases or cancer. Despite the great interest in tetraspanins, the structural and biochemical basis of their activity is still largely unknown. A major bottleneck lies in the difficulty of obtaining stable and homogeneous protein samples in large quantities. Here we report expression screening of 15 members of the human tetraspanin superfamily and successful protocols for the production in S. cerevisiae of a subset of tetraspanins involved in human cancer development. We have demonstrated the subcellular localization of overexpressed tetraspanin-green fluorescent protein fusion proteins in S. cerevisiae and found that despite being mislocalized, the fusion proteins are not degraded. The recombinantly produced tetraspanins are dispersed within the endoplasmic reticulum membranes or localized in granule-like structures in yeast cells. The recombinantly produced tetraspanins can be extracted from the membrane fraction and purified with detergents or the poly (styreneco-maleic acid) polymer technique for use in further biochemical or biophysical studies.
ER -
SKAAR, Karin, Henryj J. KORZA, Michael TARRY, Petra SEKYROVÁ and Martin HOGBOM. Expression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiae. \textit{Plos one}. San Francisco: Public Library of Science, 2015, vol.~10, No~7, p.~nestránkováno, 19 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0134041.
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