JANOUŠKOVÁ, Eliška, Ivona NEČASOVÁ, Jana PAVLOUŠKOVÁ, Michal ZIMMERMANN, Milan HLUCHÝ, María Victoria MARINI PALOMEQUE, Monika NOVÁKOVÁ and Ctirad HOFR. Human Rap1 modulates TRF2 attraction to telomeric DNA. Nucleic Acids Research. Oxford: Oxford University Press, 2015, vol. 43, No 5, p. 2691-2700. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkv097. |
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@article{1342280, author = {Janoušková, Eliška and Nečasová, Ivona and Pavloušková, Jana and Zimmermann, Michal and Hluchý, Milan and Marini Palomeque, María Victoria and Nováková, Monika and Hofr, Ctirad}, article_location = {Oxford}, article_number = {5}, doi = {http://dx.doi.org/10.1093/nar/gkv097}, keywords = {MAMMALIAN TELOMERES; HOLLIDAY JUNCTIONS; PROTEIN; PROTECTION; SHELTERIN; BINDING; LOOP; END; ATM; RECRUITMENT}, language = {eng}, issn = {0305-1048}, journal = {Nucleic Acids Research}, title = {Human Rap1 modulates TRF2 attraction to telomeric DNA}, url = {http://nar.oxfordjournals.org/content/43/5/2691.full.pdf+html}, volume = {43}, year = {2015} }
TY - JOUR ID - 1342280 AU - Janoušková, Eliška - Nečasová, Ivona - Pavloušková, Jana - Zimmermann, Michal - Hluchý, Milan - Marini Palomeque, María Victoria - Nováková, Monika - Hofr, Ctirad PY - 2015 TI - Human Rap1 modulates TRF2 attraction to telomeric DNA JF - Nucleic Acids Research VL - 43 IS - 5 SP - 2691-2700 EP - 2691-2700 PB - Oxford University Press SN - 03051048 KW - MAMMALIAN TELOMERES KW - HOLLIDAY JUNCTIONS KW - PROTEIN KW - PROTECTION KW - SHELTERIN KW - BINDING KW - LOOP KW - END KW - ATM KW - RECRUITMENT UR - http://nar.oxfordjournals.org/content/43/5/2691.full.pdf+html L2 - http://nar.oxfordjournals.org/content/43/5/2691.full.pdf+html N2 - More than two decades of genetic research have identified and assigned main biological functions of shelterin proteins that safeguard telomeres. However, a molecular mechanism of how each protein subunit contributes to the protecting function of the whole shelterin complex remains elusive. Human Repressor activator protein 1 (Rap1) forms a multifunctional complex with Telomeric Repeat binding Factor 2 (TRF2). Rap1-TRF2 complex is a critical part of shelterin as it suppresses homology-directed repair in Ku 70/80 heterodimer absence. To understand how Rap1 affects key functions of TRF2, we investigated full-length Rap1 binding to TRF2 and Rap1-TRF2 complex interactions with double-stranded DNA by quantitative biochemical approaches. We observed that Rap1 reduces the overall DNA duplex binding affinity of TRF2 but increases the selectivity of TRF2 to telomeric DNA. Additionally, we observed that Rap1 induces a partial release of TRF2 from DNA duplex. The improved TRF2 selectivity to telomeric DNA is caused by less pronounced electrostatic attractions between TRF2 and DNA in Rap1 presence. Thus, Rap1 prompts more accurate and selective TRF2 recognition of telomeric DNA and TRF2 localization on single/double-strand DNA junctions. These quantitative functional studies contribute to the understanding of the selective recognition of telomeric DNA by the whole shelterin complex. ER -
JANOUŠKOVÁ, Eliška, Ivona NEČASOVÁ, Jana PAVLOUŠKOVÁ, Michal ZIMMERMANN, Milan HLUCHÝ, María Victoria MARINI PALOMEQUE, Monika NOVÁKOVÁ and Ctirad HOFR. Human Rap1 modulates TRF2 attraction to telomeric DNA. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2015, vol.~43, No~5, p.~2691-2700. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkv097.
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