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@article{1342356,
author = {Varga, Marian and Pantůček, Roman and Růžičková, Vladislava and Doškař, Jiří},
article_location = {Reading},
article_number = {January},
doi = {http://dx.doi.org/10.1099/jgv.0.000329},
keywords = {Staphylococcus aureus; bacteriophage transduction; horizontal gene transfer; antimicrobial drug resistance; bacteriophage genomics},
language = {eng},
issn = {0022-1317},
journal = {Journal of General Virology},
title = {Molecular characterization of a new efficiently transducing bacteriophage identified in meticillin-resistant Staphylococcus aureus},
url = {http://dx.doi.org/10.1099/jgv.0.000329},
volume = {97},
year = {2016}
}
TY - JOUR
ID - 1342356
AU - Varga, Marian - Pantůček, Roman - Růžičková, Vladislava - Doškař, Jiří
PY - 2016
TI - Molecular characterization of a new efficiently transducing bacteriophage identified in meticillin-resistant Staphylococcus aureus
JF - Journal of General Virology
VL - 97
IS - January
SP - 258-267
EP - 258-267
PB - Society of General Microbiology
SN - 00221317
KW - Staphylococcus aureus
KW - bacteriophage transduction
KW - horizontal gene transfer
KW - antimicrobial drug resistance
KW - bacteriophage genomics
UR - http://dx.doi.org/10.1099/jgv.0.000329
L2 - http://dx.doi.org/10.1099/jgv.0.000329
N2 - In Staphylococcus aureus, generalized transduction mediated by temperate bacteriophages represents a highly efficient way of transferring antibiotic resistance genes between strains. In the present study, we identified and characterized in detail a new efficiently transducing bacteriophage of the family Siphoviridae, designated phiJB, which resides as a prophage in the meticillin-resistant S. aureus (MRSA) strain Jevons B. Whole-genome sequencing followed by detailed in silico analysis uncovered a linear dsDNA genome consisting of 43012 bp and comprising 70 ORFs, of which approximately 40 encoded proteins with unknown function. A global genome alignment of phiJB and other efficiently transducing phages phi11, phi53, phi80, phi80alpha and phiNM4 showed a high degree of homology with phiNM4 and substantial differences with regard to other phages. Using a model transduction system with a well-defined donor and recipient, phiJB transferred the tetracycline resistance plasmid pT181 and a penicillinase plasmid with outstanding frequencies, beating most of the above-mentioned phages by an order of magnitude. Moreover, phiJB demonstrated high frequencies of transferring antibiotic resistance plasmids even upon induction from a lysogenic donor strain. Considering such transducing potential, phiJB and related bacteriophages may serve as a suitable tool for elucidating the nature of transduction and its contribution to the spread of antibiotic resistance genes in naturally occurring MRSA populations.
ER -
VARGA, Marian, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jiří DOŠKAŘ. Molecular characterization of a new efficiently transducing bacteriophage identified in meticillin-resistant Staphylococcus aureus. \textit{Journal of General Virology}. Reading: Society of General Microbiology, 2016, vol.~97, January, p.~258-267. ISSN~0022-1317. doi:10.1099/jgv.0.000329.
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