| ŠEDO, Ondrej, Michal KOŘÁN, Michaela JAKEŠOVÁ, Renata MIKULÍKOVÁ, Michal BOHÁČ and Zbyněk ZDRÁHAL. Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry. Food Chemistry. Oxford: ELSEVIER SCI LTD, 2016, vol. 206, September, p. 124-130. ISSN 0308-8146. Available from: https://dx.doi.org/10.1016/j.foodchem.2016.03.056. |
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@article{1343703,
author = {Šedo, Ondrej and Kořán, Michal and Jakešová, Michaela and Mikulíková, Renata and Boháč, Michal and Zdráhal, Zbyněk},
article_location = {Oxford},
article_number = {September},
doi = {http://dx.doi.org/10.1016/j.foodchem.2016.03.056},
keywords = {Barley; Malting; MALDI-TOF MS; Proteins},
language = {eng},
issn = {0308-8146},
journal = {Food Chemistry},
title = {Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry},
url = {http://www.sciencedirect.com/science/article/pii/S0308814616304095},
volume = {206},
year = {2016}
}
TY - JOUR
ID - 1343703
AU - Šedo, Ondrej - Kořán, Michal - Jakešová, Michaela - Mikulíková, Renata - Boháč, Michal - Zdráhal, Zbyněk
PY - 2016
TI - Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry
JF - Food Chemistry
VL - 206
IS - September
SP - 124-130
EP - 124-130
PB - ELSEVIER SCI LTD
SN - 03088146
KW - Barley
KW - Malting
KW - MALDI-TOF MS
KW - Proteins
UR - http://www.sciencedirect.com/science/article/pii/S0308814616304095
L2 - http://www.sciencedirect.com/science/article/pii/S0308814616304095
N2 - A method for discriminating malting barley varieties based on direct matrix-assisted laser desorption-ionisation -time-of-flight mass spectrometry (MALDI-TOF MS) fingerprinting of proteins was developed. Signals corresponding to hordeins were obtained by simple mixing of powdered barley grain with a MALDI matrix solution containing 12.5 mg mL(-1) of ferulic acid in an acetonitrile: water: formic acid 50:33:17 v/v/v mixture. Compared to previous attempts at MALDI-TOF mass spectrometric analysis of barley proteins, the extraction and fractionation steps were practically omitted, resulting in a significant reduction in analytical time and costs. The discriminatory power was examined on twenty malting barley varieties and the practicability of the method was tested on sixty barley samples acquired from Pilsner Urquell Brewery. The method is proposed as a rapid tool for variety assignment and purity determination of malting barley that may replace gel electrophoresis currently used for this purpose.
ER -
ŠEDO, Ondrej, Michal KOŘÁN, Michaela JAKEŠOVÁ, Renata MIKULÍKOVÁ, Michal BOHÁČ and Zbyněk ZDRÁHAL. Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry. \textit{Food Chemistry}. Oxford: ELSEVIER SCI LTD, 2016, vol.~206, September, p.~124-130. ISSN~0308-8146. Available from: https://dx.doi.org/10.1016/j.foodchem.2016.03.056.
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