k 2016

MALDI TOF MS imaging of 3D neuroblastoma cell cultures

MACHÁLKOVÁ, Markéta, Adam PRUŠKA, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA, Jan PREISLER et. al.

Basic information

Original name

MALDI TOF MS imaging of 3D neuroblastoma cell cultures

Name in Czech

MALDI TOF MS zobrazování 3D neuroblastomových buněčných kultur

Authors

MACHÁLKOVÁ, Markéta (203 Czech Republic, belonging to the institution), Adam PRUŠKA (203 Czech Republic, belonging to the institution), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

5. Konference České společnosti pro hmotnostní spektrometrii, 2016

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/16:00087892

Organization unit

Faculty of Science

ISBN

978-80-905045-6-1

Keywords in English

MS; imaging; spheroid; 3D neuroblastoma cell culture; perifosine; metaiodobenzylguanidine; matrix deposition
Změněno: 19/12/2016 17:17, prof. Mgr. Jan Preisler, Ph.D.

Abstract

V originále

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206. Initial images showing distribution of the drugs and selected lipids with CHCA and DHB matrices were acquired.

Links

GA15-05387S, research and development project
Name: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic
Investor: Czech Science Foundation
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR