Detailed Information on Publication Record
2016
MALDI TOF MS imaging of 3D neuroblastoma cell cultures
MACHÁLKOVÁ, Markéta, Adam PRUŠKA, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA, Jan PREISLER et. al.Basic information
Original name
MALDI TOF MS imaging of 3D neuroblastoma cell cultures
Name in Czech
MALDI TOF MS zobrazování 3D neuroblastomových buněčných kultur
Authors
MACHÁLKOVÁ, Markéta (203 Czech Republic, belonging to the institution), Adam PRUŠKA (203 Czech Republic, belonging to the institution), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)
Edition
5. Konference České společnosti pro hmotnostní spektrometrii, 2016
Other information
Language
English
Type of outcome
Prezentace na konferencích
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/16:00087892
Organization unit
Faculty of Science
ISBN
978-80-905045-6-1
Keywords in English
MS; imaging; spheroid; 3D neuroblastoma cell culture; perifosine; metaiodobenzylguanidine; matrix deposition
Změněno: 19/12/2016 17:17, prof. Mgr. Jan Preisler, Ph.D.
Abstract
V originále
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206. Initial images showing distribution of the drugs and selected lipids with CHCA and DHB matrices were acquired.
Links
GA15-05387S, research and development project |
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LQ1601, research and development project |
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