MALDI TOF MS imaging of 3D neuroblastoma cell cultures

MACHÁLKOVÁ, Markéta, Adam PRUŠKA, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA and Jan PREISLER. MALDI TOF MS imaging of 3D neuroblastoma cell cultures. In 5. Konference České společnosti pro hmotnostní spektrometrii. 2016. ISBN 978-80-905045-6-1.

Basic information

• Original name: MALDI TOF MS imaging of 3D neuroblastoma cell cultures
• Name in Czech: MALDI TOF MS zobrazování 3D neuroblastomových buněčných kultur
• Authors: MACHÁLKOVÁ, Markéta (203 Czech Republic, belonging to the institution), Adam PRUŠKA (203 Czech Republic, belonging to the institution), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 5. Konference České společnosti pro hmotnostní spektrometrii, 2016.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/16:00087892
• Organization unit: Faculty of Science
• ISBN: 978-80-905045-6-1
• Keywords in English: MS; imaging; spheroid; 3D neuroblastoma cell culture; perifosine; metaiodobenzylguanidine; matrix deposition

Abstract

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206. Initial images showing distribution of the drugs and selected lipids with CHCA and DHB matrices were acquired.

Links

• GA15-05387S, research and development project: Name: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

Investor: Czech Science Foundation

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR