Detailed Information on Publication Record
2016
Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil
SZÁKOVÁ, Jiřina, Jitka HAVLÍČKOVÁ, Adéla ŠÍPKOVÁ, Jiří GABRIEL, Karel ŠVEC et. al.Basic information
Original name
Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil
Authors
SZÁKOVÁ, Jiřina (203 Czech Republic, guarantor), Jitka HAVLÍČKOVÁ (203 Czech Republic), Adéla ŠÍPKOVÁ (203 Czech Republic), Jiří GABRIEL (203 Czech Republic), Karel ŠVEC (203 Czech Republic), Petr BALDRIAN (203 Czech Republic), Jiřina SYSALOVÁ (203 Czech Republic), Pavel COUFALÍK (203 Czech Republic, belonging to the institution), Rostislav ČERVENKA (203 Czech Republic, belonging to the institution), Ondřej ZVĚŘINA (203 Czech Republic, belonging to the institution), Josef KOMÁREK (203 Czech Republic, belonging to the institution) and Pavel TLUSTOŠ (203 Czech Republic)
Edition
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Philadelphia, Taylor & Francis, 2016, 1093-4529
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.425
RIV identification code
RIV/00216224:14310/16:00087915
Organization unit
Faculty of Science
UT WoS
000369491300011
Keywords in English
mercury; microbial community; mobility; rhizobox; speciation
Tags
International impact, Reviewed
Změněno: 15/12/2016 13:11, Ing. Mgr. Věra Pospíšilíková
Abstract
V originále
The precise characterization of the behavior of individual microorganisms in the presence of increased mercury contents in soil is necessary for better elucidation of the fate of mercury in the soil environment. In our investigation, resistant bacterial strains isolated from two mercury contaminated soils, represented by Paenibacillus alginolyticus, Burkholderia glathei, Burkholderia sp., and Pseudomonas sp., were used. Two differently contaminated soils (0.5 and 7mg kg(-1) total mercury) were chosen. Preliminary soil analysis showed the presence of methylmercury and phenylmercury with the higher soil mercury level. Modified rhizobox experiments were performed to assess the ability of mercury accumulating strains to deplete the mobile and mobilizable mercury portions in the soil by modification; microbial agar cultures were used rather than the plant root zone. A sequential extraction procedure was performed to release the following mercury fractions: water soluble, extracted in acidic conditions, bound to humic substances, elemental, and bound to complexes, HgS and residual. Inductively coupled plasma mass spectrometry (ICP-MS) and a single-purpose atomic absorption spectrometer (AMA-254) were applied for mercury determination in the samples and extracts. Gas chromatography coupled to atomic fluorescence spectrometry (GC-AFS) was used for the determination of organomercury compounds. The analysis of the microbial community at the end of the experiment showed a 42% abundance of Paenibacillus sp. followed by Acetivibrio sp., Brevibacillus sp., Cohnella sp., Lysinibacillus sp., and Clostridium sp. not exceeding 2% abundance. The results suggest importance of Paenibacillus sp. in Hg transformation processes. This genus should be tested for potential bioremediation use in further research.
Links
| GAP503/12/0682, research and development project |
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