DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)

NOREK, Adam, Lubomír JANDA and Alena ŽÁKOVSKÁ. DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic). Journal of Vector Ecology. Wiley, 2016, vol. 41, No 1, p. 172-178. ISSN 1081-1710. Available from: https://dx.doi.org/10.1111/jvec.12209.

Basic information

• Original name: DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)
• Authors: NOREK, Adam (203 Czech Republic, belonging to the institution), Lubomír JANDA (203 Czech Republic, belonging to the institution) and Alena ŽÁKOVSKÁ (203 Czech Republic, belonging to the institution).
• Edition: Journal of Vector Ecology, Wiley, 2016, 1081-1710.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.473
• RIV identification code: RIV/00216224:14310/16:00089869
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1111/jvec.12209
• UT WoS: 000377219600023
• Keywords (in Czech): Borrelia burgdorferi sensu lato OspC FlaB
• Keywords in English: Borrelia burgdorferi sensu lato OspC FlaB
• Tags: AKR, rivok
• Tags: International impact, Reviewed

Abstract

In Europe nine different genospecies responsible for the human form of the LD have been reported. Among those B. afzelii, B. garinii and B. burdgorferii s. s. are the most prevalent in the Czech Republic. Each genospecies can be further subdivided into specific genotypes depending on genetic marker used in analysis. In our study we used two different loci (flaB and ospC) to assign genospecies and OspC phylogenetic type to eighteen strains previously isolated from ticks collected at a locality Pisárky, a suburban park near the city of Brno (Czech Republic). The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii and B. valaisiana). Three samples from collection contained more than one genospecies. For other fifteen strains nucleotide sequences of flaB and ospC were determined. The phylogenetic analysis assigned our isolates to B. garinii (12) and B. afzelii (3) respectively, which were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, G5 (B. garinii) and A3 and A8 (B. afzelii).

Links

• MUNI/E/0131/2009, interní kód MU: Name: Sekvenace kandidátního genu pro vakcínu, tj. genu pro OspC protein u českcýh izolátů kmenů patogenní Borrelia burgdorferi sensu lato, původce Lymeské borreliózy

Investor: Masaryk University, Category E - development project + specific research