DNA-based identification and OspC serotyping in cultures of
Borrelia burgdorferi s.l. isolated from ticks collected in the
Moravia (Czech Republic)

J 2016

DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)

NOREK, Adam, Lubomír JANDA and Alena ŽÁKOVSKÁ

Basic information

Original name

DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)

Authors

NOREK, Adam (203 Czech Republic, belonging to the institution), Lubomír JANDA (203 Czech Republic, belonging to the institution) and Alena ŽÁKOVSKÁ (203 Czech Republic, belonging to the institution)

Edition

Journal of Vector Ecology, Wiley, 2016, 1081-1710

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.473

RIV identification code

RIV/00216224:14310/16:00089869

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/jvec.12209

UT WoS

000377219600023

Keywords (in Czech)

Borrelia burgdorferi sensu lato OspC FlaB

Keywords in English

Borrelia burgdorferi sensu lato OspC FlaB

Abstract

V originále

In Europe nine different genospecies responsible for the human form of the LD have been reported. Among those B. afzelii, B. garinii and B. burdgorferii s. s. are the most prevalent in the Czech Republic. Each genospecies can be further subdivided into specific genotypes depending on genetic marker used in analysis. In our study we used two different loci (flaB and ospC) to assign genospecies and OspC phylogenetic type to eighteen strains previously isolated from ticks collected at a locality Pisárky, a suburban park near the city of Brno (Czech Republic). The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii and B. valaisiana). Three samples from collection contained more than one genospecies. For other fifteen strains nucleotide sequences of flaB and ospC were determined. The phylogenetic analysis assigned our isolates to B. garinii (12) and B. afzelii (3) respectively, which were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, G5 (B. garinii) and A3 and A8 (B. afzelii).

Links

MUNI/E/0131/2009, interní kód MU

Name: Sekvenace kandidátního genu pro vakcínu, tj. genu pro OspC protein u českcýh izolátů kmenů patogenní Borrelia burgdorferi sensu lato, původce Lymeské borreliózy

Investor: Masaryk University, Category E - development project + specific research

Citovat

NOREK, Adam, Lubomír JANDA and Alena ŽÁKOVSKÁ. DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic). Journal of Vector Ecology. Wiley, 2016, vol. 41, No 1, p. 172-178. ISSN 1081-1710. Available from: https://dx.doi.org/10.1111/jvec.12209.

@article{1344729,
   author = {Norek, Adam and Janda, Lubomír and Žákovská, Alena},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/jvec.12209},
   keywords = {Borrelia burgdorferi sensu lato OspC FlaB},
   language = {eng},
   issn = {1081-1710},
   journal = {Journal of Vector Ecology},
   title = {DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)},
   volume = {41},
   year = {2016}
}

TY  - JOUR
ID  - 1344729
AU  - Norek, Adam - Janda, Lubomír - Žákovská, Alena
PY  - 2016
TI  - DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)
JF  - Journal of Vector Ecology
VL  - 41
IS  - 1
SP  - 172-178
EP  - 172-178
PB  - Wiley
SN  - 10811710
KW  - Borrelia burgdorferi sensu lato OspC FlaB
N2  - In Europe nine different genospecies responsible for the human form of the LD have been reported. Among those B. afzelii, B. garinii and B. burdgorferii s. s. are the most prevalent in the Czech Republic. Each genospecies can be further subdivided into specific genotypes depending on genetic marker used in analysis. In our study we used two different loci (flaB and ospC) to assign genospecies and OspC phylogenetic type to eighteen strains previously isolated from ticks collected at a locality Pisárky, a suburban park near the city of Brno (Czech Republic). The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii and B. valaisiana). Three samples from collection contained more than one genospecies. For other fifteen strains nucleotide sequences of flaB and ospC were determined. The phylogenetic analysis assigned our isolates to B. garinii (12) and B. afzelii (3) respectively, which were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, G5 (B. garinii) and A3 and A8 (B. afzelii).
ER  -

NOREK, Adam, Lubomír JANDA and Alena ŽÁKOVSKÁ. DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic). \textit{Journal of Vector Ecology}. Wiley, 2016, vol.~41, No~1, p.~172-178. ISSN~1081-1710. Available from: https://dx.doi.org/10.1111/jvec.12209.

Detailed Information on Publication Record