Toxoplasma gondii and Neospora caninum in wild small mammals:
seroprevalence, DNA detection and genotyping

J 2016

Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping

MACHAČOVÁ, Tereza, Daniel AJZENBERG, Alena ŽÁKOVSKÁ, Kamil SEDLÁK, Eva BÁRTOVÁ et. al.

Basic information

Original name

Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping

Authors

MACHAČOVÁ, Tereza (203 Czech Republic, guarantor), Daniel AJZENBERG (250 France), Alena ŽÁKOVSKÁ (203 Czech Republic, belonging to the institution), Kamil SEDLÁK (203 Czech Republic) and Eva BÁRTOVÁ (203 Czech Republic)

Edition

Veterinary Parasitology, Elsevier Science, 2016, 0304-4017

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.356

RIV identification code

RIV/00216224:14310/16:00089871

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.vetpar.2016.04.018

UT WoS

000377317200015

Keywords (in Czech)

Toxoplasmosis neosporosis hlodavci genotyp

Keywords in English

Toxoplasmosis neosporosis rodents genotype Czech Republic

Abstract

V originále

Generally, rodents and other small mammals are considered as one of the sources of Toxoplasma gondii or Neospora caninum infection for cats and dogs as the definitive hosts of these two parasites, respectively. The aim of the study was to find out the prevalence of these two parasites in wild small mammals from the Czech Republic and to characterize T. gondii isolates by methods of molecular biology. A total of 621 wild small mammals were caught in the Czech Republic during years 2002 – 2014. Antibodies to T. gondii were detected by latex agglutination test in six (2.5 percent) of 240 small mammals (in two A. agrarius and four A. flavicollis). Antibodies to N. caninum were detected by commercially available competitive-inhibition enzyme-linked immunosorbent assay (percent inhibition value is more than 30 percent) in one A. flavicolis (0.4 percent). Three of 427 (0.7 percent) liver samples were positive for T. gondii by PCR while negative for N. caninum. All embryo samples (n = 102) were negative for both T. gondii and N. caninum. Three T. gondii isolates (two A. flavicollis and one A. sylvaticus) were genotyped by microsatellite markers and characterized as type II. To our knowledge, this is the first information about genetic characterization of T. gondii isolates in small mammals from Europe and the first detection of N. caninum antibodies in wild rodents from the Czech Republic.

Citovat

MACHAČOVÁ, Tereza, Daniel AJZENBERG, Alena ŽÁKOVSKÁ, Kamil SEDLÁK and Eva BÁRTOVÁ. Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping. Veterinary Parasitology. Elsevier Science, 2016, vol. 223, June, p. 88-90. ISSN 0304-4017. Available from: https://dx.doi.org/10.1016/j.vetpar.2016.04.018.

@article{1344738,
   author = {Machačová, Tereza and Ajzenberg, Daniel and Žákovská, Alena and Sedlák, Kamil and Bártová, Eva},
   article_number = {June},
   doi = {http://dx.doi.org/10.1016/j.vetpar.2016.04.018},
   keywords = {Toxoplasmosis neosporosis rodents genotype Czech Republic},
   language = {eng},
   issn = {0304-4017},
   journal = {Veterinary Parasitology},
   title = {Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping},
   volume = {223},
   year = {2016}
}

TY  - JOUR
ID  - 1344738
AU  - Machačová, Tereza - Ajzenberg, Daniel - Žákovská, Alena - Sedlák, Kamil - Bártová, Eva
PY  - 2016
TI  - Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping
JF  - Veterinary Parasitology
VL  - 223
IS  - June
SP  - 88-90
EP  - 88-90
PB  - Elsevier Science
SN  - 03044017
KW  - Toxoplasmosis neosporosis rodents genotype Czech Republic
N2  - Generally, rodents and other small mammals are considered as one of the sources of Toxoplasma gondii or Neospora caninum infection for cats and dogs as the definitive hosts of these two parasites, respectively. The aim of the study was to find out the prevalence of these two parasites in wild small mammals from the Czech Republic and to characterize T. gondii isolates by methods of molecular biology. A total of 621 wild small mammals were caught in the Czech Republic during years 2002 – 2014. Antibodies to T. gondii were detected by latex agglutination test in six (2.5 percent) of 240 small mammals (in two A. agrarius and four A. flavicollis). Antibodies to N. caninum were detected by commercially available competitive-inhibition enzyme-linked immunosorbent assay (percent inhibition value is more than 30 percent) in one A. flavicolis (0.4 percent). Three of 427 (0.7 percent) liver samples were positive for T. gondii by PCR while negative for N. caninum. All embryo samples (n = 102) were negative for both T. gondii and N. caninum. Three T. gondii isolates (two A. flavicollis and one A. sylvaticus) were genotyped by microsatellite markers and characterized as type II. To our knowledge, this is the first information about genetic characterization of T. gondii isolates in small mammals from Europe and the first detection of N. caninum antibodies in wild rodents from the Czech Republic.
ER  -

MACHAČOVÁ, Tereza, Daniel AJZENBERG, Alena ŽÁKOVSKÁ, Kamil SEDLÁK and Eva BÁRTOVÁ. Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping. \textit{Veterinary Parasitology}. Elsevier Science, 2016, vol.~223, June, p.~88-90. ISSN~0304-4017. Available from: https://dx.doi.org/10.1016/j.vetpar.2016.04.018.

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