MACHAČOVÁ, Tereza, Daniel AJZENBERG, Alena ŽÁKOVSKÁ, Kamil SEDLÁK and Eva BÁRTOVÁ. Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping. Veterinary Parasitology. Elsevier Science, 2016, vol. 223, June, p. 88-90. ISSN 0304-4017. Available from: https://dx.doi.org/10.1016/j.vetpar.2016.04.018.
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Basic information
Original name Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping
Authors MACHAČOVÁ, Tereza (203 Czech Republic, guarantor), Daniel AJZENBERG (250 France), Alena ŽÁKOVSKÁ (203 Czech Republic, belonging to the institution), Kamil SEDLÁK (203 Czech Republic) and Eva BÁRTOVÁ (203 Czech Republic).
Edition Veterinary Parasitology, Elsevier Science, 2016, 0304-4017.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.356
RIV identification code RIV/00216224:14310/16:00089871
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.vetpar.2016.04.018
UT WoS 000377317200015
Keywords (in Czech) Toxoplasmosis neosporosis hlodavci genotyp
Keywords in English Toxoplasmosis neosporosis rodents genotype Czech Republic
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 14/4/2017 20:58.
Abstract
Generally, rodents and other small mammals are considered as one of the sources of Toxoplasma gondii or Neospora caninum infection for cats and dogs as the definitive hosts of these two parasites, respectively. The aim of the study was to find out the prevalence of these two parasites in wild small mammals from the Czech Republic and to characterize T. gondii isolates by methods of molecular biology. A total of 621 wild small mammals were caught in the Czech Republic during years 2002 – 2014. Antibodies to T. gondii were detected by latex agglutination test in six (2.5 percent) of 240 small mammals (in two A. agrarius and four A. flavicollis). Antibodies to N. caninum were detected by commercially available competitive-inhibition enzyme-linked immunosorbent assay (percent inhibition value is more than 30 percent) in one A. flavicolis (0.4 percent). Three of 427 (0.7 percent) liver samples were positive for T. gondii by PCR while negative for N. caninum. All embryo samples (n = 102) were negative for both T. gondii and N. caninum. Three T. gondii isolates (two A. flavicollis and one A. sylvaticus) were genotyped by microsatellite markers and characterized as type II. To our knowledge, this is the first information about genetic characterization of T. gondii isolates in small mammals from Europe and the first detection of N. caninum antibodies in wild rodents from the Czech Republic.
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