Electrochemical and spectral behaviour of miR-34a-5p

J 2016

Electrochemical and spectral behaviour of miR-34a-5p

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK, Libuše TRNKOVÁ et. al.

Basic information

Original name

Electrochemical and spectral behaviour of miR-34a-5p

Authors

VEČEŘOVÁ, Aneta (203 Czech Republic, belonging to the institution), Kristýna HUDCOVÁ (203 Czech Republic, belonging to the institution), Iveta PILAŘOVÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Monatshefte fur Chemie, Wien, Springer, 2016, 0026-9247

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.282

RIV identification code

RIV/00216224:14110/16:00089932

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00706-015-1600-y

UT WoS

000367521400014

Keywords in English

miR-34a-5p; Human cancer; Voltammetry; Circular dichroic spectroscopy; Mercury electrode

Abstract

V originále

MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA -> miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3-6. [GRAPHICS] .

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

LH13053, research and development project

Name: Chemická a elektrochemická analýza purinových derivátů a jejich komplexů s mědí (Acronym: CECAP)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK and Libuše TRNKOVÁ. Electrochemical and spectral behaviour of miR-34a-5p. Monatshefte fur Chemie. Wien: Springer, 2016, vol. 147, No 1, p. 105-110. ISSN 0026-9247. Available from: https://dx.doi.org/10.1007/s00706-015-1600-y.

@article{1345275,
   author = {Večeřová, Aneta and Hudcová, Kristýna and Pilařová, Iveta and Masařík, Michal and Trnková, Libuše},
   article_location = {Wien},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s00706-015-1600-y},
   keywords = {miR-34a-5p; Human cancer; Voltammetry; Circular dichroic spectroscopy; Mercury electrode},
   language = {eng},
   issn = {0026-9247},
   journal = {Monatshefte fur Chemie},
   title = {Electrochemical and spectral behaviour of miR-34a-5p},
   url = {http://link.springer.com/article/10.1007%2Fs00706-015-1600-y},
   volume = {147},
   year = {2016}
}

TY  - JOUR
ID  - 1345275
AU  - Večeřová, Aneta - Hudcová, Kristýna - Pilařová, Iveta - Masařík, Michal - Trnková, Libuše
PY  - 2016
TI  - Electrochemical and spectral behaviour of miR-34a-5p
JF  - Monatshefte fur Chemie
VL  - 147
IS  - 1
SP  - 105-110
EP  - 105-110
PB  - Springer
SN  - 00269247
KW  - miR-34a-5p
KW  - Human cancer
KW  - Voltammetry
KW  - Circular dichroic spectroscopy
KW  - Mercury electrode
UR  - http://link.springer.com/article/10.1007%2Fs00706-015-1600-y
L2  - http://link.springer.com/article/10.1007%2Fs00706-015-1600-y
N2  - MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA -> miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3-6. [GRAPHICS] .
ER  -

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK and Libuše TRNKOVÁ. Electrochemical and spectral behaviour of miR-34a-5p. \textit{Monatshefte fur Chemie}. Wien: Springer, 2016, vol.~147, No~1, p.~105-110. ISSN~0026-9247. Available from: https://dx.doi.org/10.1007/s00706-015-1600-y.

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