Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics:
Preparation, Characterization and Medical Applications

J 2016

Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications

ABDEL-RAHMAN, Rasha M., A. M. ABDEL-MOHSEN, Radim HRDINA, Ladislav BURGERT, Zdenka FOHLEROVÁ et. al.

Basic information

Original name

Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications

Authors

ABDEL-RAHMAN, Rasha M. (818 Egypt, guarantor), A. M. ABDEL-MOHSEN (818 Egypt), Radim HRDINA (203 Czech Republic), Ladislav BURGERT (203 Czech Republic), Zdenka FOHLEROVÁ (203 Czech Republic), David PAVLIŇÁK (203 Czech Republic, belonging to the institution), O.N. SAYED (818 Egypt) and Josef JANČÁŘ (203 Czech Republic)

Edition

International Journal of Biological Macromolecules, Elsevier, 2016, 0141-8130

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10305 Fluids and plasma physics

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

odkaz na článek

Impact factor

Impact factor: 3.671

RIV identification code

RIV/00216224:14310/16:00089952

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ijbiomac.2016.04.087

UT WoS

000378951400086

Keywords in English

Hyaluronan;chitosan;nonwoven fabrics;cytotoxicity;healing properties

Abstract

V originále

Thin layers of chitosan (positively charged)/ sodium hyaluronate (negatively charged)/ nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/ nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different tools such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E.coli) and Streptococcus aureus (St. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics 4 interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.

Links

ED2.1.00/03.0086, research and development project

Name: Regionální VaV centrum pro nízkonákladové plazmové a nanotechnologické povrchové úpravy

LO1411, research and development project

Name: Rozvoj centra pro nízkonákladové plazmové a nanotechnologické povrchové úpravy (Acronym: CEPLANT plus)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

ABDEL-RAHMAN, Rasha M., A. M. ABDEL-MOHSEN, Radim HRDINA, Ladislav BURGERT, Zdenka FOHLEROVÁ, David PAVLIŇÁK, O.N. SAYED and Josef JANČÁŘ. Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications. International Journal of Biological Macromolecules. Elsevier, 2016, vol. 89, August, p. 725-736. ISSN 0141-8130. Available from: https://dx.doi.org/10.1016/j.ijbiomac.2016.04.087.

@article{1345393,
   author = {AbdelandRahman, Rasha M. and AbdelandMohsen, A. M. and Hrdina, Radim and Burgert, Ladislav and Fohlerová, Zdenka and Pavliňák, David and Sayed, O.N. and Jančář, Josef},
   article_number = {August},
   doi = {http://dx.doi.org/10.1016/j.ijbiomac.2016.04.087},
   keywords = {Hyaluronan;chitosan;nonwoven fabrics;cytotoxicity;healing properties},
   language = {eng},
   issn = {0141-8130},
   journal = {International Journal of Biological Macromolecules},
   title = {Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications},
   url = {http://www.sciencedirect.com/science/article/pii/S0141813016304044},
   volume = {89},
   year = {2016}
}

TY  - JOUR
ID  - 1345393
AU  - Abdel-Rahman, Rasha M. - Abdel-Mohsen, A. M. - Hrdina, Radim - Burgert, Ladislav - Fohlerová, Zdenka - Pavliňák, David - Sayed, O.N. - Jančář, Josef
PY  - 2016
TI  - Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications
JF  - International Journal of Biological Macromolecules
VL  - 89
IS  - August
SP  - 725-736
EP  - 725-736
PB  - Elsevier
SN  - 01418130
KW  - Hyaluronan;chitosan;nonwoven fabrics;cytotoxicity;healing properties
UR  - http://www.sciencedirect.com/science/article/pii/S0141813016304044
L2  - http://www.sciencedirect.com/science/article/pii/S0141813016304044
N2  - Thin layers of chitosan (positively charged)/ sodium hyaluronate (negatively charged)/ nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/ nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different tools such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E.coli) and Streptococcus aureus (St. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics 4 interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.
ER  -

ABDEL-RAHMAN, Rasha M., A. M. ABDEL-MOHSEN, Radim HRDINA, Ladislav BURGERT, Zdenka FOHLEROVÁ, David PAVLIŇÁK, O.N. SAYED and Josef JANČÁŘ. Wound Dressing based on Chitosan/hyaluronan/Nonwoven Fabrics: Preparation, Characterization and Medical Applications. \textit{International Journal of Biological Macromolecules}. Elsevier, 2016, vol.~89, August, p.~725-736. ISSN~0141-8130. Available from: https://dx.doi.org/10.1016/j.ijbiomac.2016.04.087.

Detailed Information on Publication Record