A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA
Analysis: Soluble Bound BMAA Found to Be an Important Fraction

J 2016

A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction

FAASSEN, Elisabeth J., Maria G. ANTONIOU, Wendy BEEKMAN-LUKASSEN, Lucie BLÁHOVÁ, Ekaterina CHERNOVA et. al.

Basic information

Original name

A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction

Authors

FAASSEN, Elisabeth J. (528 Netherlands), Maria G. ANTONIOU (196 Cyprus), Wendy BEEKMAN-LUKASSEN (528 Netherlands), Lucie BLÁHOVÁ (203 Czech Republic, guarantor, belonging to the institution), Ekaterina CHERNOVA (643 Russian Federation), Christophoros CHRISTOPHORIDIS (300 Greece), Audrey COMBES (250 France), Christine EDWARDS (826 United Kingdom of Great Britain and Northern Ireland), Jutta FASTNER (276 Germany), Joop HARMSEN (528 Netherlands), Anastasia HISKIA (300 Greece), Leopold L. ILAG (752 Sweden), Triantafyllos KALOUDIS (300 Greece), Srdjan LOPICIC (688 Serbia), Miquel LURLING (528 Netherlands), Hanna MAZUR-MARZEC (616 Poland), Jussi MERILUOTO (246 Finland), Cristina POROJAN (372 Ireland), Yehudit VINER-MOZZINI (376 Israel) and Nadezda ZGUNA (752 Sweden)

Edition

MARINE DRUGS, BASEL, MDPI AG, 2016, 1660-3397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.503

RIV identification code

RIV/00216224:14310/16:00089982

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/md14030045

UT WoS

000373701200014

Keywords in English

6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC); seafood; phytoplankton; Daphnia magna; Liquid chromatography-tandem mass spectrometry (LC-MS-MS); beta-N-methylamino-l-alanine (BMAA); Internal standard

Abstract

V originále

Exposure to beta-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D(3)BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D(3)BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.

Citovat

FAASSEN, Elisabeth J., Maria G. ANTONIOU, Wendy BEEKMAN-LUKASSEN, Lucie BLÁHOVÁ, Ekaterina CHERNOVA, Christophoros CHRISTOPHORIDIS, Audrey COMBES, Christine EDWARDS, Jutta FASTNER, Joop HARMSEN, Anastasia HISKIA, Leopold L. ILAG, Triantafyllos KALOUDIS, Srdjan LOPICIC, Miquel LURLING, Hanna MAZUR-MARZEC, Jussi MERILUOTO, Cristina POROJAN, Yehudit VINER-MOZZINI and Nadezda ZGUNA. A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction. MARINE DRUGS. BASEL: MDPI AG, 2016, vol. 14, No 3, p. 1-12. ISSN 1660-3397. Available from: https://dx.doi.org/10.3390/md14030045.

@article{1345763,
   author = {Faassen, Elisabeth J. and Antoniou, Maria G. and BeekmanandLukassen, Wendy and Bláhová, Lucie and Chernova, Ekaterina and Christophoridis, Christophoros and Combes, Audrey and Edwards, Christine and Fastner, Jutta and Harmsen, Joop and Hiskia, Anastasia and Ilag, Leopold L. and Kaloudis, Triantafyllos and Lopicic, Srdjan and Lurling, Miquel and MazurandMarzec, Hanna and Meriluoto, Jussi and Porojan, Cristina and VinerandMozzini, Yehudit and Zguna, Nadezda},
   article_location = {BASEL},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/md14030045},
   keywords = {6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC); seafood; phytoplankton; Daphnia magna; Liquid chromatography-tandem mass spectrometry (LC-MS-MS); beta-N-methylamino-l-alanine (BMAA); Internal standard},
   language = {eng},
   issn = {1660-3397},
   journal = {MARINE DRUGS},
   title = {A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction},
   url = {http://www.mdpi.com/1660-3397/14/3/45},
   volume = {14},
   year = {2016}
}

TY  - JOUR
ID  - 1345763
AU  - Faassen, Elisabeth J. - Antoniou, Maria G. - Beekman-Lukassen, Wendy - Bláhová, Lucie - Chernova, Ekaterina - Christophoridis, Christophoros - Combes, Audrey - Edwards, Christine - Fastner, Jutta - Harmsen, Joop - Hiskia, Anastasia - Ilag, Leopold L. - Kaloudis, Triantafyllos - Lopicic, Srdjan - Lurling, Miquel - Mazur-Marzec, Hanna - Meriluoto, Jussi - Porojan, Cristina - Viner-Mozzini, Yehudit - Zguna, Nadezda
PY  - 2016
TI  - A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction
JF  - MARINE DRUGS
VL  - 14
IS  - 3
SP  - 1-12
EP  - 1-12
PB  - MDPI AG
SN  - 16603397
KW  - 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC)
KW  - seafood
KW  - phytoplankton
KW  - Daphnia magna
KW  - Liquid chromatography-tandem mass spectrometry (LC-MS-MS)
KW  - beta-N-methylamino-l-alanine (BMAA)
KW  - Internal standard
UR  - http://www.mdpi.com/1660-3397/14/3/45
L2  - http://www.mdpi.com/1660-3397/14/3/45
N2  - Exposure to beta-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D(3)BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D(3)BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.
ER  -

FAASSEN, Elisabeth J., Maria G. ANTONIOU, Wendy BEEKMAN-LUKASSEN, Lucie BLÁHOVÁ, Ekaterina CHERNOVA, Christophoros CHRISTOPHORIDIS, Audrey COMBES, Christine EDWARDS, Jutta FASTNER, Joop HARMSEN, Anastasia HISKIA, Leopold L. ILAG, Triantafyllos KALOUDIS, Srdjan LOPICIC, Miquel LURLING, Hanna MAZUR-MARZEC, Jussi MERILUOTO, Cristina POROJAN, Yehudit VINER-MOZZINI and Nadezda ZGUNA. A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction. \textit{MARINE DRUGS}. BASEL: MDPI AG, 2016, vol.~14, No~3, p.~1-12. ISSN~1660-3397. Available from: https://dx.doi.org/10.3390/md14030045.

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