Freeze-drying as suitable method to achieve ready-to-use yeast
biosensors for androgenic and estrogenic compounds

J 2016

Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds

JARQUE ORTIZ, Sergio, Michal BITTNER and Klára HILSCHEROVÁ

Basic information

Original name

Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds

Authors

JARQUE ORTIZ, Sergio (724 Spain, belonging to the institution), Michal BITTNER (203 Czech Republic, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Chemosphere, OXFORD, Elsevier Science, 2016, 0045-6535

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.208

RIV identification code

RIV/00216224:14310/16:00089983

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chemosphere.2016.01.038

UT WoS

000371099700024

Keywords in English

Recombinant yeast assay; Freeze-drying; Estrogen; Androgen; Long-term; Ready-to-use

Abstract

V originále

Recombinant yeast assays (RYAS) have been proved to be a suitable tool for the fast screening of compounds with endocrine disrupting activities. However, ready-to-use versions more accessible to less equipped laboratories and field studies are scarce and far from optimal throughputs. Here, we have applied freeze-drying technology to optimize RYA for the fast assessment of environmental compounds with estrogenic and androgenic potencies. The effects of different cryoprotectants, initial optical density and long-term storage were evaluated. The study included detailed characterization of sensitivity, robustness and reproducibility of the new ready-to-use versions, as well as comparison with the standard assays. Freeze-dried RYAS showed similar dose-responses curves to their homolog standard assays, with Lowest Observed Effect Concentration (LOEC) and Median effective Concentration (EC50) of 1 nM and 7.5 nM for testosterone, and 0.05 nM and 0.5 nM for 17 beta-estradiol, respectively. Freeze-dried cells stored at 4 degrees C retained maximum sensitivity up to 2 months, while cells stored at -18 degrees C showed no decrease in sensitivity throughout the study (10 months). This ready-to-use RYA is easily accessible and may be potentially used for on-site applications.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

JARQUE ORTIZ, Sergio, Michal BITTNER and Klára HILSCHEROVÁ. Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds. Chemosphere. OXFORD: Elsevier Science, 2016, vol. 148, April, p. 204-210. ISSN 0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2016.01.038.

@article{1345764,
   author = {Jarque Ortiz, Sergio and Bittner, Michal and Hilscherová, Klára},
   article_location = {OXFORD},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2016.01.038},
   keywords = {Recombinant yeast assay; Freeze-drying; Estrogen; Androgen; Long-term; Ready-to-use},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds},
   url = {http://www.sciencedirect.com/science/article/pii/S0045653516300376},
   volume = {148},
   year = {2016}
}

TY  - JOUR
ID  - 1345764
AU  - Jarque Ortiz, Sergio - Bittner, Michal - Hilscherová, Klára
PY  - 2016
TI  - Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds
JF  - Chemosphere
VL  - 148
IS  - April
SP  - 204-210
EP  - 204-210
PB  - Elsevier Science
SN  - 00456535
KW  - Recombinant yeast assay
KW  - Freeze-drying
KW  - Estrogen
KW  - Androgen
KW  - Long-term
KW  - Ready-to-use
UR  - http://www.sciencedirect.com/science/article/pii/S0045653516300376
L2  - http://www.sciencedirect.com/science/article/pii/S0045653516300376
N2  - Recombinant yeast assays (RYAS) have been proved to be a suitable tool for the fast screening of compounds with endocrine disrupting activities. However, ready-to-use versions more accessible to less equipped laboratories and field studies are scarce and far from optimal throughputs. Here, we have applied freeze-drying technology to optimize RYA for the fast assessment of environmental compounds with estrogenic and androgenic potencies. The effects of different cryoprotectants, initial optical density and long-term storage were evaluated. The study included detailed characterization of sensitivity, robustness and reproducibility of the new ready-to-use versions, as well as comparison with the standard assays. Freeze-dried RYAS showed similar dose-responses curves to their homolog standard assays, with Lowest Observed Effect Concentration (LOEC) and Median effective Concentration (EC50) of 1 nM and 7.5 nM for testosterone, and 0.05 nM and 0.5 nM for 17 beta-estradiol, respectively. Freeze-dried cells stored at 4 degrees C retained maximum sensitivity up to 2 months, while cells stored at -18 degrees C showed no decrease in sensitivity throughout the study (10 months). This ready-to-use RYA is easily accessible and may be potentially used for on-site applications.
ER  -

JARQUE ORTIZ, Sergio, Michal BITTNER and Klára HILSCHEROVÁ. Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds. \textit{Chemosphere}. OXFORD: Elsevier Science, 2016, vol.~148, April, p.~204-210. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2016.01.038.

Detailed Information on Publication Record