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@article{1345765,
author = {Ouyang, Xiyu and Leonards, Pim E. G. and Toušová, Zuzana and Slobodník, Jaroslav and de Boer, Jacob and Lamoreet, Marja H.},
article_location = {WASHINGTON},
article_number = {4},
doi = {http://dx.doi.org/10.1021/acs.analchem.5b04311},
keywords = {2-DIMENSIONAL LIQUID-CHROMATOGRAPHY; CARBAMATE INSECTICIDES; TOXICANTS; MIXTURES; POTENCY; SAMPLES},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry},
url = {http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04311},
volume = {88},
year = {2016}
}
TY - JOUR
ID - 1345765
AU - Ouyang, Xiyu - Leonards, Pim E. G. - Toušová, Zuzana - Slobodník, Jaroslav - de Boer, Jacob - Lamoreet, Marja H.
PY - 2016
TI - Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry
JF - Analytical Chemistry
VL - 88
IS - 4
SP - 2353-2360
EP - 2353-2360
PB - AMER CHEMICAL SOC
SN - 00032700
KW - 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY
KW - CARBAMATE INSECTICIDES
KW - TOXICANTS
KW - MIXTURES
KW - POTENCY
KW - SAMPLES
UR - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04311
L2 - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04311
N2 - Effect-directed analysis (EDA) is a useful tool to identify bioactive compounds in complex samples. However, identification in EDA is usually challenging, mainly due to limited separation power of the liquid chromatography based fractionation. In this study, comprehensive two-dimensional liquid chromatography (LC X LC) based microfractionation combined with parallel high resolution time of flight (HR-ToF) mass spectrometric detection and a high throughput acetylcholinesterase (AChE) assay was developed. The LC x LC fractionation method was validated using analytical standards and a C18 and pentafluorophenyl (PFP) stationary phase combination was selected for the two-dimensional separation and fractionation in four 96-well plates. The method was successfully applied to identify AChE inhibitors in a wastewater treatment plant (WWTP) effluent. Good orthogonality (>0.9) separation was achieved and three AChE inhibitors (tiapride, amisulpride, and lamotrigine), used as antipsychotic medicines, were identified and confirmed by two-dimensional retention alignment as well as their AChE inhibition activity.
ER -
OUYANG, Xiyu, Pim E. G. LEONARDS, Zuzana TOUŠOVÁ, Jaroslav SLOBODNÍK, Jacob DE BOER and Marja H. LAMOREET. Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry. \textit{Analytical Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2016, vol.~88, No~4, p.~2353-2360. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.5b04311.
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