Rapid Screening of Acetylcholinesterase Inhibitors by
Effect-Directed Analysis Using LC x LC Fractionation, a High
Throughput in Vitro Assay, and Parallel Identification by Time
of Flight Mass Spectrometry

J 2016

Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry

OUYANG, Xiyu, Pim E. G. LEONARDS, Zuzana TOUŠOVÁ, Jaroslav SLOBODNÍK, Jacob DE BOER et. al.

Basic information

Original name

Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry

Authors

OUYANG, Xiyu (528 Netherlands), Pim E. G. LEONARDS (528 Netherlands), Zuzana TOUŠOVÁ (203 Czech Republic, guarantor, belonging to the institution), Jaroslav SLOBODNÍK (703 Slovakia), Jacob DE BOER (528 Netherlands) and Marja H. LAMOREET (528 Netherlands)

Edition

Analytical Chemistry, WASHINGTON, AMER CHEMICAL SOC, 2016, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.320

RIV identification code

RIV/00216224:14310/16:00089984

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acs.analchem.5b04311

UT WoS

000370454000053

Keywords in English

2-DIMENSIONAL LIQUID-CHROMATOGRAPHY; CARBAMATE INSECTICIDES; TOXICANTS; MIXTURES; POTENCY; SAMPLES

Abstract

V originále

Effect-directed analysis (EDA) is a useful tool to identify bioactive compounds in complex samples. However, identification in EDA is usually challenging, mainly due to limited separation power of the liquid chromatography based fractionation. In this study, comprehensive two-dimensional liquid chromatography (LC X LC) based microfractionation combined with parallel high resolution time of flight (HR-ToF) mass spectrometric detection and a high throughput acetylcholinesterase (AChE) assay was developed. The LC x LC fractionation method was validated using analytical standards and a C18 and pentafluorophenyl (PFP) stationary phase combination was selected for the two-dimensional separation and fractionation in four 96-well plates. The method was successfully applied to identify AChE inhibitors in a wastewater treatment plant (WWTP) effluent. Good orthogonality (>0.9) separation was achieved and three AChE inhibitors (tiapride, amisulpride, and lamotrigine), used as antipsychotic medicines, were identified and confirmed by two-dimensional retention alignment as well as their AChE inhibition activity.

Citovat

OUYANG, Xiyu, Pim E. G. LEONARDS, Zuzana TOUŠOVÁ, Jaroslav SLOBODNÍK, Jacob DE BOER and Marja H. LAMOREET. Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry. Analytical Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2016, vol. 88, No 4, p. 2353-2360. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.5b04311.

@article{1345765,
   author = {Ouyang, Xiyu and Leonards, Pim E. G. and Toušová, Zuzana and Slobodník, Jaroslav and de Boer, Jacob and Lamoreet, Marja H.},
   article_location = {WASHINGTON},
   article_number = {4},
   doi = {http://dx.doi.org/10.1021/acs.analchem.5b04311},
   keywords = {2-DIMENSIONAL LIQUID-CHROMATOGRAPHY; CARBAMATE INSECTICIDES; TOXICANTS; MIXTURES; POTENCY; SAMPLES},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry},
   url = {http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04311},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1345765
AU  - Ouyang, Xiyu - Leonards, Pim E. G. - Toušová, Zuzana - Slobodník, Jaroslav - de Boer, Jacob - Lamoreet, Marja H.
PY  - 2016
TI  - Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry
JF  - Analytical Chemistry
VL  - 88
IS  - 4
SP  - 2353-2360
EP  - 2353-2360
PB  - AMER CHEMICAL SOC
SN  - 00032700
KW  - 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY
KW  - CARBAMATE INSECTICIDES
KW  - TOXICANTS
KW  - MIXTURES
KW  - POTENCY
KW  - SAMPLES
UR  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04311
L2  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04311
N2  - Effect-directed analysis (EDA) is a useful tool to identify bioactive compounds in complex samples. However, identification in EDA is usually challenging, mainly due to limited separation power of the liquid chromatography based fractionation. In this study, comprehensive two-dimensional liquid chromatography (LC X LC) based microfractionation combined with parallel high resolution time of flight (HR-ToF) mass spectrometric detection and a high throughput acetylcholinesterase (AChE) assay was developed. The LC x LC fractionation method was validated using analytical standards and a C18 and pentafluorophenyl (PFP) stationary phase combination was selected for the two-dimensional separation and fractionation in four 96-well plates. The method was successfully applied to identify AChE inhibitors in a wastewater treatment plant (WWTP) effluent. Good orthogonality (>0.9) separation was achieved and three AChE inhibitors (tiapride, amisulpride, and lamotrigine), used as antipsychotic medicines, were identified and confirmed by two-dimensional retention alignment as well as their AChE inhibition activity.
ER  -

OUYANG, Xiyu, Pim E. G. LEONARDS, Zuzana TOUŠOVÁ, Jaroslav SLOBODNÍK, Jacob DE BOER and Marja H. LAMOREET. Rapid Screening of Acetylcholinesterase Inhibitors by Effect-Directed Analysis Using LC x LC Fractionation, a High Throughput in Vitro Assay, and Parallel Identification by Time of Flight Mass Spectrometry. \textit{Analytical Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2016, vol.~88, No~4, p.~2353-2360. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.5b04311.

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