| KRUPKA, Michal, Josef MASEK, Lucia BARKOCZIOVA, Pavlina Turanek KNOTIGOVA, Pavel KULICH, Jana PLOCKOVA, Robert LUKAC, Eliska BARTHELDYOVA, Stepan KOUDELKA, Radka CHALOUPKOVÁ, Marek SEBELA, Daniel ZYKA, Ladislav DROZ, Roman EFFENBERG, Miroslav LEDVINA, Andrew D. MILLER, Jaroslav TURANEK a Milan RASKA. The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice. Plos one. San Francisco: Public Library of Science, roč. 11, č. 2, s. "nestrankovano", 17 s. ISSN 1932-6203. doi:10.1371/journal.pone.0148497. 2016. |
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@article{1345767,
author = {Krupka, Michal and Masek, Josef and Barkocziova, Lucia and Knotigova, Pavlina Turanek and Kulich, Pavel and Plockova, Jana and Lukac, Robert and Bartheldyova, Eliska and Koudelka, Stepan and Chaloupková, Radka and Sebela, Marek and Zyka, Daniel and Droz, Ladislav and Effenberg, Roman and Ledvina, Miroslav and Miller, Andrew D. and Turanek, Jaroslav and Raska, Milan},
article_location = {San Francisco},
article_number = {2},
doi = {http://dx.doi.org/10.1371/journal.pone.0148497},
keywords = {OUTER SURFACE PROTEIN; LYME-DISEASE VACCINE; BORRELIA-BURGDORFERI OSPA; LIPOPHILIC DERIVATIVES; MOLECULAR ANALYSIS; IMMUNE-RESPONSE; GENE-TRANSFER; EPITOPE; EXPRESSION; ANTIGEN},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice},
url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148497},
volume = {11},
year = {2016}
}
TY - JOUR
ID - 1345767
AU - Krupka, Michal - Masek, Josef - Barkocziova, Lucia - Knotigova, Pavlina Turanek - Kulich, Pavel - Plockova, Jana - Lukac, Robert - Bartheldyova, Eliska - Koudelka, Stepan - Chaloupková, Radka - Sebela, Marek - Zyka, Daniel - Droz, Ladislav - Effenberg, Roman - Ledvina, Miroslav - Miller, Andrew D. - Turanek, Jaroslav - Raska, Milan
PY - 2016
TI - The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice
JF - Plos one
VL - 11
IS - 2
SP - "nestrankovano"
EP - "nestrankovano"
PB - Public Library of Science
SN - 19326203
KW - OUTER SURFACE PROTEIN
KW - LYME-DISEASE VACCINE
KW - BORRELIA-BURGDORFERI OSPA
KW - LIPOPHILIC DERIVATIVES
KW - MOLECULAR ANALYSIS
KW - IMMUNE-RESPONSE
KW - GENE-TRANSFER
KW - EPITOPE
KW - EXPRESSION
KW - ANTIGEN
UR - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148497
L2 - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148497
N2 - Lyme disease, Borrelia burgdorferi-caused infection, if not recognized and appropriately treated by antibiotics, may lead to chronic complications, thus stressing the need for protective vaccine development. The immune protection is mediated by phagocytic cells and by Borrelia-specific complement-activating antibodies, associated with the Th1 immune response. Surface antigen OspC is involved in Borrelia spreading through the host body. Previously we reported that recombinant histidine tagged (His-tag) OspC (rOspC) could be attached onto liposome surfaces by metallochelation. Here we report that levels of OspC-specific antibodies vary substantially depending upon whether rOspC possesses an N' or C' terminal His-tag. This is the case in mice immunized: (a) with rOspC proteoliposomes containing adjuvants MPLA or non-pyrogenic MDP analogue MT06; (b) with free rOspC and Montanide PET GEL A; (c) with free rOspC and alum; or (d) with adjuvant-free rOspC. Stronger responses are noted with all N'-terminal His-tag rOspC formulations. OspC-specific Th1-type antibodies predominate post-immunization with rOspC proteoliposomes formulated with MPLA or MT06 adjuvants. Further analyses confirmed that the structural features of soluble N' and C' terminal His-tag rOspC and respective rOspC proteoliposomes are similar including their thermal stabilities at physiological temperatures. On the other hand, a change in the position of the rOspC His-tag from N' to C' terminal appears to affect substantially the immunogenicity of rOspC arguably due to steric hindrance of OspC epitopes by the C' terminal His-tag itself and not due to differences in overall conformations induced by changes in the His-tag position in rOspC variants.
ER -
KRUPKA, Michal, Josef MASEK, Lucia BARKOCZIOVA, Pavlina Turanek KNOTIGOVA, Pavel KULICH, Jana PLOCKOVA, Robert LUKAC, Eliska BARTHELDYOVA, Stepan KOUDELKA, Radka CHALOUPKOVÁ, Marek SEBELA, Daniel ZYKA, Ladislav DROZ, Roman EFFENBERG, Miroslav LEDVINA, Andrew D. MILLER, Jaroslav TURANEK a Milan RASKA. The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice. \textit{Plos one}. San Francisco: Public Library of Science, roč.~11, č.~2, s.~''nestrankovano'', 17 s. ISSN~1932-6203. doi:10.1371/journal.pone.0148497. 2016.
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